Amino Acids Proteins, and Enzymes Types of Proteins Amino Acids The Peptide Bond
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Types of Proteins • • • • • • •
Type Structural Contractile Transport Storage Hormonal Enzyme Protection
Examples tendons, cartilage, hair, nails muscles hemoglobin milk insulin, growth hormone catalyzes reactions in cells immune response 2
Amino Acids • • • •
Building blocks of proteins Carboxylic acid group Amino group Side group R gives unique characteristics R side chain I H2H—C —COOH I H
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Examples of Amino Acids H I H2N—C —COOH I H glycine CH3 I H2N—C —COOH I H alanine
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Types of Amino Acids Nonpolar R = H, CH3, alkyl groups, aromatic O Polar ll R = –CH2OH, –CH2SH, –CH2C–NH2, (polar groups with –O-, -SH, -N-) Polar/Acidic R = –CH2COOH, or -COOH Polar/ Basic R = –CH2CH2NH2
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Learning Check AA1 Identify each as (1) polar or (2) nonpolar A. NH2–CH2–COOH
(Glycine)
CH3 | CH–OH | B. NH2–CH–COOH
(Serine) 6
Solution AA1 Identify each as (1) polar or (2) nonpolar A.(2) NH2–CH2–COOH
(Glycine)
CH3 | CH–OH | B. (1) NH2–CH–COOH
(Serine)
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Essential Amino Acids • 10 amino acids not synthesized by the body • arg, his, ile, leu, lys, met, phe, thr, trp, val • Must obtain from the diet • All in diary products • 1 or more missing in grains and vegetables 8
Amino Acids as Acids and Bases • Ionization of the –NH2 and the –COOH group • Zwitterion has both a + and – charge • Zwitterion is neutral overall + NH2–CH2–COOH glycine
H3N–CH2–COO– Zwitterion of glycine 9
pH and ionization H+ +
OH– +
H3N–CH2–COOH
H3N–CH2–COO–
H2N–CH2–COO–
Positive ion
zwitterion
Negative ion
Low pH
neutral pH
High pH 10
Learning Check AA2 CH3
CH3
+ H3N–CH–COOH (1)
H2N–CH2–COO– (2)
Select from the above structures A. Alanine in base. B. Alanine in acid. 11
Solution AA2 CH3
CH3
+ H3N–CH–COOH (1)
H2N–CH2–COO– (2)
Select from the above structures •
(2) Alanine in base.
•
(1) Alanine in acid. 12
The Peptide Bond Amide bond formed by the –COOH of an amino acid and the –NH2 of the next amino acid O ||
+
CH3 + |
NH3–CH2–COH +
+
H3N–CH–COO–
O
CH3
||
|
NH3–CH2–C – N–CH–COO– | H
peptide bond 13
Peptides • Amino acids linked by amide (peptide) bonds Gly
Lys
Phe
H2Nend
Arg
Ser -COOH
Peptide bonds
end
Glycyllysylphenylalanylarginylserine 14
Learning Check AA3 What are the possible tripeptides formed from one each of leucine, glycine, and alanine?
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Solution AA3 Tripeptides possible from one each of leucine, glycine, and alanine Leu-Gly-Ala Leu-Ala-Gly Ala-Leu-Gly Ala-Gly-Leu Gly-Ala-Leu Gly-Leu-Ala 16
Learning Check AA4 Write the three-letter abbreviations for the following tetrapeptide: CH3 CH3 CH3 O
S
CH CH3
SH
CH2
CH O
CH2 O
CH2 O -
H3N CH C N CH C N CH C N CH C O H
H
H
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Solution AA4 CH3 CH 3 CH 3 O
S
CH CH 3
SH
CH2
CH O
CH 2 O
CH2 O
H 3N CH C N CH C N CH C N CH C O
H
H
H
Ala-Leu-Cys-Met 18