7a102-mt----molecular Biology & Virology

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Code No: 7A102/MT M.Tech. – I Semester Supplementary Examinations, September, 2008 MOLECULAR BIOLOGY & VIROLOGY (Biotechnology) Time: 3hours

Max. Marks:60 Answer any FIVE questions All questions carry equal marks ---

1.

Answer the following questions:a) Describe different classes of RNAs and their functions briefly? b) What are the Post – Transcriptional Modification required for t RNA maturation?

2.

Answer the following questions:a) Repetitive DNA types and its significance b) Mechanism of Gene amplification.

3.

What are transposable elements? Describe the structure and their mode of transposition from one location to another in the genome?

4.

How proximal and distal transcription in Eukaryotes?

5.

Answer the following questions:a) Explain the mechanism of spliceosome mediated splicing of RNA? b) How antisense molecules used in the inhibition of RNA splicing?

6.

Describe the mechanism of Gene silencing using siRNA? Highlight applications of RNAi technology?

7.

Describe the methods adopted in the classification of viruses? Highlight the Baltimore system of virus classification?

8.

Describe the following stages in Animal virus replication? a) Attachment and entry of virion b) Integration and replication of viral genetic material. c) Budding, Maturation and release of virus.

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