7a101-mt----microbial Engineering

NR

Code No: 7A101/MT M.Tech. – I Semester Supplementary Examinations, September, 2008 MICROBIAL ENGINEERING (Biotechnology) Time: 3hours

1.a) b) 2.a) b) c) 3.a)

b) 4.a) b)

5.a) b)

Max. Marks:60

Answer any FIVE questions All questions carry equal marks --There is a paradigm change in the processes of production of bulk chemicals from, fossil (petroleum and coal) resources to renewable resources - Enumerate. Write on the evolution of (chronological development) of modern industrial (white) biotechnology. What is sterilisation? Write on its importance. Write on the effect of dosage of toxic chemicals and the time of exposure of these chemicals on microbes. Explain various airfilters used for elimination of microorganisms. The over all reaction for microbial conversion of glucose to glutamic acid is: (not balanced reaction) C6 H12O6 + NH 3 + O2 → C5 H 9 NO4 + CO2 + H 2O What mass of oxygen is required to produce 15gm of glutamic acid? Cell is an open system and violates 2nd law of thermodynamics – enumerate. Define doubling time and number of generations. What is the relation between them? The following data is obtained from a chemostat fermentation studies. A feed containing substrate at a concentration of 0.5 kg / m3 at a rate of 111cc/sec is fed into 2.5 m3 volume of reactor. If the Ks = 50 gm/cc and µm =12 min −1 find the conversion of substrate. Discuss on the role of maintenance and maintenance coefficient during the substrate utilization. Write in detail on the different phases of growth of microorganism in batch culture. Contd….2

Code No: 7A101/MT

::2::

6.

What is a model in the context of microbial growth? Explain the differences and similarities of compartmental and metabolic growth models.

7.

Distinguish between fermentation reactions classification by a) Gaden and b) Deindoerfer. Explain with the help of figures.

8.

State and explain the various production parameters considered for production of recombinant Hepatitis-B vaccine or any other rDNA product. ******