Use Of Hptlc, Hplc, And Densitometry For Qualitative Separation Of Indole Alkaloids From Rauvolfia Serpent In A Roots [m. M. Gupta, Alpana Srivastava Arvind K. Tripathi, Himanshu Misra, R. K. Verma]

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Use of HPTLC, HPLC, andAdd to marked items Add to shopping cart densitometry for qualitativeAdd to saved items separation of indole alkaloids fromRecommend this article Rauvolfia serpentina roots

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JPC - Journal of Planar Chromatography Modern TLC Akadémiai Kiadó 0933-4173 (Print) 17890993 (Online) Volume 19, Number 110/August 2006 Original Research Papers 10.1556/JPC.19.2006.4.5 282-287 Wednesday, March 07, 2007

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Within all content Within this journal Madan Mohan Gupta , Alpana Srivastava , Arvind Kumar Tripathi , Himanshu Misra ,Within this issue Ram Kishore Verma1 Export this article Export this article as RIS | 1 Central Institute of Medicinal and Aromatic Plants Analytical Chemistry Division P.O.Text CIMAP Lucknow 226015 India 1

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Abstract High-performance thin-layer chromatography (HPTLC) has been used for normalphase separation of the components of hexane, chloroform, methanol, and water extracts of Rauvolfia serpentina roots. Computerized densitometry was used for twodimensional spectrographic image analysis of the HPTLC plates. High performance liquid chromatography (HPLC) was also used for reversed-phase separation of these extracts. Different chromatograms of R. serpentina root extracts, obtained by use of these techniques, revealed the presence of three marker indole alkaloids, ajmaline, ajmalicine, and reserpine, in all four extracts. Use of chloroform resulted in most efficient extraction of these three alkaloids. The results also showed that defatting with hexane may result in loss of the alkaloids. Keywords HPTLC, HPLC, Densitometry, Rauvolfia serpentina, Alkaloids References H.J. Pfeifer, D.J. Greenblatt , and J. Koch-Weser , Am. J. Med. Sci. 271 (1976) 269–

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