Unit 1-jan13
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Chapter 18: Structure of DNA
• • •
Replication is only for DNA
•
Due to protein varying so much, it was thought in the 1800’s to be genetic code
•
Timeline of DNA
DNA is transcribed into RNA RNA is translated into protein
○
Late 20’s: Griffith Transformation Experiment
○
Transformation is uptake of naked DNA into cell
Conclusion: the bacteria can pass something on
40’s: Hershey and Chase Experiment
Used a bacteriophage
•
Combined with radioisotopes
○ ○ •
P will show up in DNA S will show up protein
35
Put mixture of each in a blender (Isotope, phage, and host)
○ ○ ○
P and 35S
32
32
In
32
In
35
P, supernatant is hot S, pellet is hot
Chargoff
Analyzed DNA from different species
Percentages differ with base pairs but stay the same within species
Also discovered that G amount = C amount and T amount = A amount
1/13/2009 10:27 PM
• • •
Replication is only for DNA
•
Due to protein varying so much, it was thought in the 1800’s to be genetic code
•
Timeline of DNA
DNA is transcribed into RNA RNA is translated into protein
○
Late 20’s: Griffith Transformation Experiment
○
Transformation is uptake of naked DNA into cell
Conclusion: the bacteria can pass something on
40’s: Hershey and Chase Experiment
Used a bacteriophage
•
Combined with radioisotopes
○ ○ •
P will show up in DNA S will show up protein
35
Put mixture of each in a blender (Isotope, phage, and host)
○ ○ ○
P and 35S
32
32
In
32
In
35
P, supernatant is hot S, pellet is hot
Chargoff
Analyzed DNA from different species
Percentages differ with base pairs but stay the same within species
Also discovered that G amount = C amount and T amount = A amount
1/13/2009 10:27 PM
