Syllabus Ptu Biotech

PTU/BOS/BSBT/111/10-01-2008/batch-2006

Punjab Technical University, Jalandhar. B.Sc Biotechnology Study Scheme

1st Semester S. No. 1. 2. 3. 4. 5. 6. 7. 8. 9. 10

2

nd

Course No. BSBT101 BSBT103 BSBT105 BSBT107 BSBT09(M) BSBT109(B) BSBT111 BSBT113 BSBT115 BSBT117 Total

Internal Marks

External Marks

Total Marks

L

T

P

2

-

-

40

60

100

3

1

-

40

60

100

Introduction & Fundamentals of Biotechnology Computer Application in Biotechnology Basic Math & Biostat

3

1

-

40

60

100

3

1

-

40

60

100

4

-

-

40

60

100

Basics of Biosciences

3

-

-

20

30

50

Inorganic Chemistry Lab

-

-

2

40

60

100

Introduction & Fundamentals of Biotechnology Lab Basics of Biosciences Lab

-

-

2

40

60

100

-

-

4

20

30

50

-

-

2

40

60

100

1 8

3

1 0

L

T

P

Course Title Technical Writing Communication Skills Inorganic Chemistry

&

Computer Application Biotechnology Lab

in

900

Semester Internal Marks

External Marks

Total Marks

S. N o. 1.

Course No.

Course Title

BSBT-102

Advance Life Sciences

3

-

-

40

60

100

2.

BSBT-104

General Microbiology

3

-

-

40

60

100

3.

BSBT-106

Physical Chemistry

3

-

-

40

60

100

4.

BSBT-108

Biochemistry

3

-

-

40

60

100

5.

BSBT-110

Molecular Biology and Genetics

3

-

-

40

60

100

6.

BSBT-112

Advance Life Sciences Lab

-

4

40

60

100

7.

BSBT-114

General Microbiology Lab

-

-

4

40

60

100

8.

BSBT-116

Physical Chemistry Lab

-

-

4

40

60

100

9.

BSBT-118

Biochemistry Lab

-

-

4

40

60

100

10

BSBT-120

Molecular Biology and Genetics

-

-

4

40

60

100

400

600

1000

Lab Total Total contact hours =35

1

PTU/BOS/BSBT/111/10-01-2008/batch-2006

3rd Semester L S. No. 1. 2. 3. 4. 5. 7. 8. 9.

Course No. BSBT- 201 BSBT-203 BSBT-205 BSBT-207 BSBT-209 BSBT-211 BSBT-213 BSBT-215 Total

T

P

Course Title Organic Chemistry Biophysics Immunology – I Techniques in Biotechnology-I Biochemistry-II Organic Chemistry Lab Biochemistry-II Lab Immunology – I Lab

4 4 4 4 4 20

1 1 1 1 4

3 3 3 9

Internal Marks 40 40 40 40 40 40 40 40 320

External Marks 60 60 60 60 60 60 60 60 480

Total Marks 100 100 100 100 100 100 100 100 800

4th Semester L S. No. 1. 2. 3. 4.

Course No. BSBT- 202 BSBT-204 BSBT-206 BSBT-208

5. 7. 8. 9.

BSBT-210 BSBT-212 BSBT-214 BSBT-216

T

P

Course Title Immunology- II Genetics Recombinant DNA Technology Fundamentals of Industrial Biotechnology Plant Biology Immunology- II Lab Genetics lab Recombinant DNA Technology Lab

Total

2

Internal Marks

External Marks

Total Marks

3 3 3 3

-

-

40 40 40 40

60 60 60 60

100 100 100 100

3 -

-

4 4 4

40 40 40 40

60 60 60 60

100 100 100 100

15

-

12

320

480

800

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 101 Technical Writing & Communication Skills

1. Communication, its types and significance: - Communication, Process of communication its kinds, channels and role in the society. 2. Reading skills: - Process of reading, reading purposes, models, strategies methodologies, reading activities, structure of meaning techniques. 3. Writing skills: - Elements of effective writing, writing styles, scientific and technical writing. 4. Grammar: - Transformation of sentences, words used as different parts of speech, one word substitution, abbreviations, technical terms etc. 5. Business correspondence: - Business letters, elements of business writing, kinds of business letters, office order memorandum, report, purchase order, quotations and tenders, job application letters, personal resume and curriculum vitae etc. 6. Listening skills: - Process of listening, barriers to listening, effective listening skills, feedback skills. 7. Speaking skills: - Speech mechanism, organs of speech, production and classification of speech sounds, phonetic transcription, skills of effective speaking, components of an effective talk, oral presentation and the role of audio visual aids in it. 8. Discussion, meeting and telephone skills: - Group discussion, conducting a meeting, attending telephonic calls

Books Recommended 1. 2. 3.

Bhattacharya, Indrajit. An approach to Communication Skills Bansal, RK and Harrison, JB. Spoken English Wright, Chissie. Handbook of Practical Communication Skills

3

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 103 Inorganic Chemistry 1. Periodic Properties Position of elements in the periodic table, effective nuclear charge and its calculations, atomic and ionic radii, ionization energy, electron affinity and electro negativity definition, methods of determination sends in periodic table and applications in predicting and explaining the chemical behavior.

2. Chemistry of Noble gases Chemical properties of noble gases, chemistry of xenon, structure and bonding, in xenon compounds, clathrates, types and stability.

3. Chemical Bonding (a) Covalent bond, directional characteristics of covalent. (b) Valence bond theory and its limitations. (c) Various types of hybridization and shapes of inorganic molecules and ions-BeF2, SnCl2, XeF4, BF3, NH4, H2O, ClF4, ICl2, PF6, SF6 and IF7. (d) Molecular orbital theory, Homonuclear (elements and ions of 1st and 2nd row) and heteronuclear (BO, CN, CO’, NO, CO, CN’), Multicenter bonding in electron deficient molecules (BORANES). (e) Weak interactions, Hydrogen bonding & vandor walls forces. 4. Coordination compounds Introduction, Werner’s coordination theory, naming of coordination compounds, stereochemistry, Geometrical isomerism and optical isomerism in compounds having coordination number 4 and 6. 5. Bonding in metal complexes Valence bond theory, electroneutrality and back bonding, limitations of VB theory, Crystal field theory, Splitting of d orbitals, calculation of CFSE in high spin and low spin, octahedral and high spin tetrahedral complexes, thermodynamic effects of CF splitting, paramagnetism, diamagnetism, ferromagnetism and anti-ferromagnetism 6. Molecular Orbital theory, π acid complexes

Books Recommended 1) 2) 3) 4)

th

J.D. Lee, Inorganic Chemistry, 5 edition chapman & Hall, London. Inorganic Chemistry by Puri, Sharma and Kalia F.A. Cotton and G. Wilkinson, Advanced Inorganic Chemistry F. Basalo and R.C. Johson, Co-ordination Chemistry,1964.

4

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 105 Introduction & Fundamentals of Biotechnology

1. Introduction to Biotechnology: Modern Biotechnology, Branches of Biotechnology and its scope. 2. Biological systems in Biotechnology: Prokaryotic systems (E. coli, Bacillus), eukaryotic systems (Saccharomyces), mammalian and non-mammalian cells in culture, organismal systems. 3. Basic techniques in Biotechnology: a. Centrifugation (Principle, types and applications) b. Electrophoresis (Principle, support media, protein and N.S. Electrophoresis) c.

Chromatography (Principle, types and applications)

d. Lyophilization (Principle, mechanism and applications) e. Basic Microscopy (Principle, various types of microscopes and introduction to electron microscopy) f.

Radioisotopy (various types of radioisotopes and instrumentation)

g. Spectroscopy

4. Basic Genetics and Genetic manipulations: - Mendelian Inheritance, Physical basis of inheritance, Gene interactions, bacterial and viral genetic systems, Genomic and mitochondrial DNA, C value paradox, cot curve, Cytoplasmic inheritance, Nucleo-cytoplasmic interactions, Development and evolutionary genetics

Books Recommended 1. McGregor, C.W.; Membrane separation in Biotechnology; Marcel Dekker, Inc, New York. 2. Frieferder, S.; Physical Biochemistry; Freeman and Co., New York. 3. Biotol Series (I - IV); Techniques used in Bioproduct Analysis; Buterworth Heineman,U.K. 4. Gardner; Principle of genetics. 5. Work, T.S.; Lab. Techniques in Biochemistry and Molecular Biology, Elsevier, New York.

5

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 107 Computer application in Biotechnology

1. General introduction: computers, organization of computers, digital and analogue computers, computer algorithms. 2. Introduction to computers and its uses: milestones in hardware and software, batch oriented/ online/ real. 3. Computers as a system: Basic concepts, stored programs, functional units and their interrelation: communication with computer. 4. Data storage devices primary storage: storage addressed and capacity, type of memory, Secondary storage Devices, Magnetic Tape-data representation and R/W, Magnetic disks, fixed and removable, data representation and R/W: Floppy and hard disks, Optical disks CD-Rom, Mass Storage Devices. 5. Input/Output Devices: Key-tape/diskette devices, light pen mouse, joystick, source data automation, Printed outputs: serial, line, page, printers, Plotters, voice response units 6. Introduction to Bioinformatics: Internet and the Biologist, Bibliographic databases, genebank sequence database, sequence analysis using GCG, sequence alignment and database searching, Multiple sequence alignments, Phylogenetic analysis, Preiction of Protein structures, submitting DNA sequences to the database, The NCBI data model

Books Recommended 1. Bioinformatics: The Machine Learning Approach, Eds – P. Baldi and S. Brunak 2. Trends in Biotechnology: Trends guide to Bioinformatics, Trends Supplement, Elsvier Trends Journals

6

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 109(M) Basic Mathematics & Biostatistics (For Biology Students)

1) Algebra: Equations reducible to quadratics, simultaneous equations (linear and quadratic), Determinants, properties of solution of simultaneous equations by Cramer's rule, matrices, definition of special kinds of matrices, arithmetic operations on matrices, inverse of a matrix, solution of simultaneous equations by matrices, pharmaceutical applications of determinants and matrices. Mensuration and its pharmaceutical applications. 2) Measures of Central Value: Objectives and pre -requisites of an ideal, measure, mean, mode and median. 3) Trignometry: Measurement of angle, T-ratios, addition, subtraction and transformation formulae, T-ratios of multiple, submultiple, allied and certain angles .. Application of logarithms in pharmaceutical computations. 4) Analytical Plane Geometry: Certain co-ordinates, distance between two points, area of triangle, a locus of point, straight line; slope and intercept from, double-intercept form, slope-point and two point form, general equation of first degree. 5) Calculus: (a) Differential: Limits and functions, definition of differential coefficient, differentiation of standard functions, including function of a function (Chain rule). Differentiation of implicit functions, logarithmic differentiation, parametric differentiation, successive differentiation. (b) Integral: Integration as inverse of differentiation, indefinite integrals of standard forms, integration by parts, substitution and partial fractions, formal evaluation of definite integrals. 6) Statistics: Recapitulation of statistics and probability. Discrete and continuous probability distributions. Binomial, Poisson and Normal distribution, applications. Curve fitting. BOOKS RECOMMENDED

1. 2. 3. 4. 5. 6.

A Textbook of Mathematics for XI-XII Students. NCERT Publications. Vol I-IV 1991 Grewal, B.S.Higher Engineering Mathematics. Khanna Publishers, New Delhi,1990 Gupta S.P., Statistical methods, thirty second edition, Sultan chand & sons Grewal B.S., Higher Engineering Mathematics thirty seventh edition, Khanna Publishers. Jindal Aman Mathematics, second edition, Kalyani Publications. Sabharwal S.S., Applied Mathematics, third edition, Eagle Publications.

7

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 109(B) Basics of Biosciences (For Maths Students)

Unit I Diversity in the living world The living world, Biological classification, Kingdom Monera, Kingdom Protista, Kingdom Fungi, Plant kingdom, Classification of animals in general Unit II Structural organization in plants and animals Morphology of flowering plants, Anatomy of plants, structural organization in animals – animal tissues, morphology and anatomy of animals Unit III Cell structure and functions Cell – Basic unit of life Bio-molecules Cell cycle and cell division

8

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT-111 Inorganic Chemistry Lab 1. Inorganic qualitative analysis. 2. Four ions including interfering ions. 3. Volumetric Analysis. 4. Iodimetry, Iodometry, Redox titrations using Ce(SO4)2 K2Cr2O7 and KMnO4, Complexometric titrations using EDTA Ca++, Mg++, Zn++ & Ni++

BSBT-113 Introduction &Fundamentals of Biotechnology Practical

1. Introduction to instrumentation: - Centrifuges, Autoclaves, Spectrophotometers, Microscopes, Laminar hoods, incubators. 2. Centrifugation including ultra-centrifugation. 3. Polyacrylamide gel electrophoresis for proteins.

BSBT-115 Basics of Bioscience Lab

Taxonomy: a) description of flowers including floral diagram, floral formula, V.S. of flower of the representative genera of families mentioned in syllabus. b) Each student required to submit a family wise herbarium consisting of at least 20 properly pressed and mounted plants.

BSBT-117 Computer Application in Bio-Technology

1. 2. 3. 4. 5. 6. 7.

Familiarization of the computer system Loading window, closing, maximizing, icon shifting & ordering. Changing drives and searching files and understanding file extensions. Saving files, protecting and unprotecting. Formatting floppies and practice on virus recognisation and protection. Practice with control panel and file manager. Practice with MS Word, Operating and closing document, Preparation of document, setting of document, familiarization with various tools, mail- merge practice. 8. Internet Browsing.

9

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 102 ADVANCED LIFE SCIENCES

Unit-I Water relations, osmosis, transpiration, water potentials, its components, physiological and molecular adaptations in plants with respect to cold-heat-drought and salt stress. Heat shock proteins, dehydrins, late embryogenesis abundant proteins, role of different osmolytes in stress tolerance.

Unit-II Plant Pathology & epidemiology: Definition, classification, mode of transmission and control measures of plant diseases. Disease resistance host pathogen interaction. Phytoalexins, PR proteins. A detailed account of the following plant diseases with respect to causal agents, symptoms, epidemiology, disease cycle and their control measures. Black stem rust of wheat, loose smut of wheat, late and early blight of potato, False smut of rice, Bacterial blight of rice, Red rot of sugarcane. TMV of potato, Yellow vein mosaic of bhindi, Bunchy top of banana, Downy mildew of bajra. Unit-III Introduction to parasitology (pertaining to various terminologies in use). Brief account of life history, mode of infection and pathogenicity of the following pathogens with reference to man, prophylaxis and treatment, Entamaeba, Trypanosoma, Leishmania, Giardia, Trichomonas and Plasmodium. Histopathological changes in organs in relation to diseases such as livers, cirrhosis, nephrosis, tumors, cancer, AIDS.

Unit-IV Arthropod vectors of human diseases: Malaria, Yellow fever, Dengue haemorragic fever, Filariasis, Plague and Epidemic typhus, Distribution and control of the above mentioned vectors. General account of diseases such as AIDS, Hepatitis, typhoid and cholera, their occurrence and eradication programmes. General account of drug therapy and drug resistance. Basic concepts of Ecology, Definitions Ecosystems: Structure and Function, Trophic levels succession. Evolution Evidence Theories, Modern Concept and Behaviour : Genetic Basis Learning, Social Behaviour. Books recommended: nd 1. M.J.Carlile, S.C.Watkinson & G.W. Gooday (2001), The Fungi 2 Ed. Academic Press. th 2. G.N.Agios (1997), Plant Pathology 4 ed. Academic Press. 3. R.S.Mehrotra (1980) Plant Pathology Tata McGrawhill New Delhi. 4. Harrison A (2000). Principles of Medicines.

10

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 104 GENERAL MICROBIOLOGY

Unit I History of Microbiology: A. Leeuwenhoek, L.Pasteur, R.Koch, J.Lister, J.Tyndall, etc. Biogenesis vs Abiogenesis, Koch Postulates, Discovery of antibiotics. Principle of Microscopy: Bright field, Dark field, Phase contrast, Fluorescent, Electron Microscopy. Unit II Microbial classification : Bacteria, Fungi and Algae. Morphology of bacteria, viruses and fungi with major emphasis on bacterial structure specially cell wall. Gram positive and Gram negative bacteria. Microbial spores, Sporulation and germination process.

Unit III Microbial growth, nutritional biodiversity, phases of growth, generation time, growth rates, monoauxic, diauxic and synchronous growth, chemostat Microbes in extreme environment like high temperature and high/ low pH Physical and chemical agents to kill microbes, sterilization and pasteurization processes Unit IV Normal micro flora in humans/animals. Types of microbial pathogens and diseases caused by them. Microbial interactions like symbiosis and antibiosis etc. Host defense mechanism against pathogens. Nitrogen fixing microbes in agriculture. Microbial metabolism, unique pathways, photosynthesis, fermentation and its products, production of heterologous proteins in microbes.

Recommended books:th 1) Davis, B.D Dulbecco, R., Eiser, H.N. and Ginsberg, H.S. (1990). Microbiology, 4 edition, Harper and Row, Publishers, Singapore. th 2) Tortora, G.J., Funke, B.R., and Case, C.L. (1994). Microbiology: an introduction, 5 edition, the Benjamin/Cummings Publishing Company, Inc. 3) Stanier, R.Y. (1995). General Microbiology, MacMillian Press London. 4) Pelczar, M.T. (1995). Microbiology, Tata McGraw Hill Publication, New Delhi. th 5) Schegel, H.G., (1995). General microbiology 7 ed. Cambridge University Press. th 6) Prescot and Dunn (1999). Industrial Microbiology, 4 ed. By S.K Jain for CBS Publishers and Distributors. th 7) Purohit, S.S. (2000). Microbiology: Fundamentals and Applications (6 edition), Agrobios (India) th 8) Postgate, J. (2000). Microbes and Man : 4 ed, Cambridge University Press.

11

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 106 PHYSICAL CHEMISTRY Unit-I Chemical Thermodynamics: State of a system, state variables, thermodynamic equilibrium, thermodynamic properties, intensive and extensive properties, various types of processes. First law of thermodynamics, internal energy and enthalpy, change in internal energy and change in enthalpy for expansion of real and ideal gases under isothermal and adiabatic conditions for reversible and irreversible processes. Relation between Cp and Cv. Internal energy change and enthaply change in a chemical process. Hess’s Law of heat summation. Enthaply of formation, enthaply of ionisation and calculation of bond dissociation energies form thermochemical data. Second law of thermodynamics, entropy and Gibb’s free energy, Carnot’s cycle, Calculation of entropy change for reversible and irreversible processes under isothermal and non-isothermal conditions. Gibbs Hemholtz equation. Third law of thermodyanamics, Nernst heat theorem, calculation of absolute entropies of substances. Thermodynamics of simple mixtures, partial molar quantities and their significance, chemical potential, Gibbs-duhem equation, variation of chemical potential with temperature and pressure, chemical potential in a mixture of ideal gases. Meaning of chemical equilibrium, homogeneous and heterogeneous equilibrias. Thermodyanamic derivation of law of chemical equilibrium, Van’t Hoff relation, Relation between free energy change and equilibrium constants Kp, Kc and Kf. Temperature and pressure dependence of equilibrium constant. Unit-II Solutions: Definition, types of solutions, vapour pressure of solution and Raoult’s law. Factors influencing the solubility of gas in liquids, Henry’s Law. Ideal solutions, Duhem-margules equation. Distillation of ideal solutions, lever rule, vapour pressure of ideal solutions and non ideal solutions. Distillation of non-ideal solutions. Azeotropes colligative properties, lowering of vapour pressure, depression in freezing point, elevation in boiling point, osmotic pressure. Their common features and applications. Thermodynamic derivation of elevation in boiling point, depression in freezing point and osmotic pressure. Van’t Hoff factor and its application to calculate degree of association and degree of dissociation. Phase Equilibria: Definition of phase, component and degree of freedom phase rule and its thermodynamic derivation. Derivation of Clausius-Clapeyron equation and its importance in phase equilibria, phase diagrams of water system, KI water system and lead-silver system.

Unit-III Chemical Kinetics: Rate of reaction, constant factors influencing rate of reaction, order, molecularity, rate equations st nd rd for 1 order, 2 order & 3 order reactions. Methods for determination order of reaction. Half life complex reactions, consecutive reactions, parallel reactions, chain reactions and opposing reactions. Activation energy and calculation from Arhenius equation. Theories of reaction rates collision theory and transition state theory of biomolecular processes. Catalysis, acid base catalysis, enzyme catalysis including the mechanisms, Michaelis Menten equation for enzyme catalysis. Electrochemistry Specific conductance, molar conductance and their dependence on electrolyte concentration, Ionic equilibria and conductance, Essential postulates of Debye Huckel theory of strong electrolytes. Mean ionic activity coefficient and ionic strength, Transport number and its relation to ionic conductance and ionic mobility. Conductometric titrations. pH scale. Buffer solutions, salt hydrolysis, acid-base indicators.

12

PTU/BOS/BSBT/111/10-01-2008/batch-2006

Electrochemical Cells: Distinction between electrolytic and electrochemical cells, standard EMF and electrode potential, Types of electrodes, Reference electrodes, calculations of ∆G, ∆H, ∆S and equilibrium constant from EMF data, Potentiometer determination of pH, Potentiometer titrations.

Unit-IV Molecular Spectroscopy: Separation of molecular energies into translational, rotational, vibrational and electronic contributions. Relative magnitude of such energy difference. Range of electromagnetic spectrum. Absorption and emission spectra, pure rotational and vibration-rotation spectra of diatomic molecules. Rotational and vibrational Raman spectra, electronic spectra of diatomic molecules.

13

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 108 BIOCHEMISTRY Unit-I Enzymes: General properties factors affecting enzyme activity regulation of enzyme activity, steady state kinetics, first order and second order kinetics, covalent modifications, classification, nomenclature types of inhibitors, inhibitors, immobilized enzymes, Ribozymes. Unit-II Metabolism: Metabolic pathways, biochemical reaction mechanism, energy rich metabolites, interorgan metabolic pathways. Carbohydrate metabolism: Biosynthesis and degradation of carbohydrates; feed pathways for glycolysis; Pentose pathway Kreb’s Cycle: Enzymes of Kreb’s cycle, amphibolic nature of the Kreb’s cycle; regulation of Kreb’s cycle, Regulation of carbohydrate metabolism Unit-III Electron transport and Oxidative phosphorylation Mitochondrial electron transport chain, oxidative phosphorylation; regulation of ATP synthesis. Unit-IV Lipid Metabolism: Digestion and absorption Biosynthesis and degradation of fatty acids; metabolism of triacyl glycerols; cholesterol metabolism, ketonobodies. Nitrogen Metabolism: Reduction and assimilation of atmospheric nitrogen, Biosynthesis and degradation of amino acids; amino acids as precursors of heme; biogenic amines; biosynthesis of degradation of nucleic acids. Porphyrins : Translation, Transcription, Replication Books recommended: nd 1. Lehninger, A.L. Nleson, D.L. and Cox, M.M. (1993), Principles of Biochemistry, 2 Ed., Worth Publishers, New York. 2. Rawn, J.D. (1989), Biochemistry, Niel Patterson Publications, North Carolnia. th 3. Berg, Tymoezko and Stryer, L (2002), Biochemistry, 5 ed. W.H. Freman & Co. San Francisco rd 4. Voet D. and Voet, J.G. (2004), Biochemistry 3 ed. John Wiley and Sons Inc. New York. 5. Voet, D., Voet, J.G. and Pratt, C.W. (1999), Fundamentals of Biocemistry, John Wilay and Sons, New York.

14

PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT – 110 MOLECULAR BIOLOGY AND GENETICS Unit-I Nature of genetic material, nucleic acids, DNA replication Organization of Chromosomes: Genome size and complexity, the supercoiling of DNA, the structure of prokaryotic and eukaryotic chromosome, Polytene chromosomes, euchromatin and heterochromatin, satellite DNA, centromere and telomere structure. Mendelian Laws of inheritance, gene interactions. Unit-II Gene linkage and chromosome mapping: Linkage and recombination of genes in chromosomes, crossing over and its molecular mechanism, gene mapping by three point test crosses, mapping by tetrad analysis, somatic cell hybridization for gene linkage studies, recombination within genes. Unit-III Mutation: Spontaneous versus induced mutations, types of mutations, the molecular basis of mutations, mechanisms of DNA repair, mutations, frequency, correlation between mutagenicity and carcinogenicity, mutagenic agents, (chemical and physical). Basic microbial genetics: Conjugation, transduction, transformation, isolation of auxotrophs, replica plating techniques, analysis of mutations in biochemical pathway, one gene – one enzyme hypothesis. Unit-IV Extrachromosomal inheritance, mitochondrial and chloroplast genetic systems, sex linked inheritance. Population Genetics: Hardy-Weinberg equilibrium, gene and genotypic frequencies, introduction of eugenics. Books recommended: 1. Maloy, S.R. Crown, J.E., and Freifelder, D. (1994): Microbial Genetics, 2nd ed. Jones and Bartlett Publishers. rd 2. Hartl, D.L, (1994), Genetics: 3 ed., Jones and Bartlett Publishers. 3. Antherly A.G. Girton, J.R. (1999), The science of Genetics, Harcourt College, Publishers. 4. Freifelder, D. (2000). Microbial Genetics, Narosa Publishing House. th 5. Hartl, D.L. Jones, E.W. (2001), Genetics: Analysis of Genes and Genomes, 5 ed. Jones & Bartlett Publishers.

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

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1) 2) 3) 4) 5) 6) 7) 8) 9) 10) 11) 12) 13) 14) 15)

BSBT-112 ADVANCED LIFE SCIENCES LAB Estimation of relative water content of leaf Measurement of osmotic potential of different tissues by Chardokov method Study of plant pathogens Symptoms of the diseases Morbid anatomy of the plants (only those diseases are to be studied which are included in the syllabus) Preparation of blood smear showing different stages of Plasmodium Study of permanent slids and specimens of parasitic protozoans, helminth and arthropods mentioned in theory syllabus. Pathological examination of blood and urine. Blood tests: Erythrocyte sedimentation rate Bleeding time Clotting time Prothrombin time

BSBT-114 GENERAL MICROBIOLOGY LAB Aseptic techniques Cleaning of glass wares, Preparation of media, Cotton plugging and sterilization Personal hygiene Microbes from hands, Tooth-Scum and other body parts. Isolation of microorganisms from air, water and soil samples Dilution and pour plating techniques. Enumeratiuon of microorganisms-total vs viable counts. Identification of isolated bacteria Gram staining, other staining methods, metabolic characterization (e.g., IMVic) tests Growth curve of microorganisms. Antibiotics sensitivity of microbes. Using antibiotic discs. Testing of water quality Test for antibodies against given bacteria One step growth of bacteriophage. Culture from body fluids (Stool, Urine, Blood). Alcoholic and mixed acid fermentation.

Books recommended Cappuccino, J.G and Sherman, N. (1992). Microbiology: Laboratory manual 3rd Edition, Benjimin/Cummings Publishing Company.

BSBT-116 PHYSICAL CHEMISTRY LAB Physical Chemistry experiments: 1. Study of distribution law by iodine distribution between water and CC14. Given standard solution Na2S2O3. 2. Study of distribution law of benzoic acid between benzene and water. 3. Determination of adsorption isotherm of oxalic acid on charcoal. 4. Surface tension: determination of surface tension of a given liquid by Stalgmimeter. 5. Determination of viscosity of a pure liquid (acetone, ethanol, propanol, butanol, glycol) (effect of hydrogen bonding on viscosity). 6. Refractometry: Determine refractive index of a given liquid as a criterion for its purity. Benzene i.e., commercial benzene + (A.R.) acetone. 7. Polarimetry: Determine the %age composition of an optically active solution.

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

8.

Conductometry: a) Determination of cell constant b) Determination of specific and equivalent conductance of electrolyte (NaC1 and HC1). c) Precipitation titration of Na2SO4 vs BaC12. d) Neutralization titrations NaOH vs HC1 and NaOH vs CH3COOH.

9.

a) pH of buffer solution. b) Acid-base titration HC1 vs NaOH. c) Determination of ionization constant of a week acid (CH3COOH). Calorimetry: a) Determination of Heat of neutralization i) Strong acid-strong base ii) Weak acid-strong base Photometry: Verification of Lambert-Beer’s law for solution of CoC12.5H2O (in water) and K2Cr2O7 (in water).

10.

11.

BSBT-118 BIOCHEMISTRY LAB 1. 2. 3. 4. 5. 6. 7.

Estimation of α-amylase activity from saliva Assay of acid phosphatase activity Effect of temperature on enzyme activity Effect of pH on enzyme activity Determination of Km for acid phosphatase Purification of protein using salt precipitation Chromatographic methods for separation of macromolecules - Paper chromatography - Thin layer chromatography - Gel permeation chromatography

Books recommended: 1. Plumer D.T. (1998), An Introduction of Practical Biochemistry, 3rd Ed. Tata McGraw Hill Publishers Co. Ltd., New Delhi. 2. Bansal, D.D., Khardori, R & Gupta, M.M. (1985), Practical Biochemistry, Standard Publication Chandigarh.

1.

2. 3. 4. 5. 6. 7. 8. 9. 10.

BSBT-120 MOLECULAR BIOLOGY AND GENETICS LAB Demonstration of Law of segregation and Independent assortment (use of coloured beads, capsules etc.) Numericals for segregation and independent assortment. Use of Chi-square for prediction of phenotype/genotype frequencies of parents from progeny and vice-versa, Epistasis. Segregation demonstration in preserved material (Maize) Detection of Blood groups (A B O & Rh factors) Inheritance of other human characteristics, ability to tast PTC, Thiourea Calculation of variance in respect of pod length and number of seeds/pod Calculation of gene frequencies and random mating (coloured beads, capsules) Paternity disputes (blood groups) Dermatographics: Palm print taking and finger tip patterns. Preparation and study of mitosis slides from buccal mucosa and onion root tips by squash method. Preparation and study of meiosis slides from meristem tissue by squash method. Demonstration of sex chromatin from buccal smear using thionin stain

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT -201 ORGANIC CHEMISTRY

1. FUNDAMENTAL ASPECTS OF ORGANIC CHEMISTRY: Inductive effects, electrometric effects, resonance, hyper conjugation, type of reagents, electrophile and nucleophile, types of organic reactions, reaction intermediates, carbocations, carbanions, free radical carbenes with complex. 2. NOMENCLATURE AND CLASSIFICATION OF ALKYL HALIDES: Methods of formation, chemical reactions, and preparation of aryl halides. Elimination-addition mechanism (benzene mechanism (Benzene mechanism) and nucleophilic aromatic substitution reactions. Relative reactivities of alkyl halides vs allyl, vinyl and aryl halide. 3. ALCOHOLS AND PHENOLS: Nomenclature, methods of formation, physical and chemical properties (Measurement of dehydration, acidity, mechanism of Kolbe’s reaction, Reimer Tiemann reaction and mechanism). 4. CARBOXYLIC ACIDS AND DERIVATIVES: Structure of carboxylic acid and derivatives, methods of formation, physical and chemical properties. 5. ALKENES, CYCLOALKENES, DIENES AND ALKYNES: Nomenclature, methods of formation, physical and chemical properties, conformation of Alkenes and cycloalkenes. Dienes – Structure and properties, Conjugation and resonance, polymerization, structure of butadienes and allenes. Alkynes-industrial source of acetylene, physical and chemical properties, Bonding of alkynes, acidity of alkynes. 6. ALKANES AND CYCLOALKANES: Nomenclature, methods of formation, physical and chemical properties. 7. ARENES AND AROMATICITY: Nomenclature of benzene derivatives, aryl group, aromatic nucleus and side chain, structure of benzene, molecular formula, Kekule structure, stability, Carbon-carbon bond length of Benzene, resonance structure, MO picture. RECOMMENDED BOOKS 1. ORGANIC CHEMISTRY – FINAR IL 2. Organic Chemistry _ Morrison and Boyd 3. Voge’s text book of Organic Chemistry – Furniss 4. Organic Chemistry – Ege Sezham

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT-203 BIOPHYSICS

1. PHYSICAL MEASUREMENT Accuracy of measurements - quantification, errors - origin

2. 3. 4. 5. 6.

and elimination, Sensitivity of measurements, Experimental data processing, Electronic methods of measurement in Biophysics. FUNDAMENTALS OF CONTROL THEORY: Concepts and definitions, Feedback systems Modeling and identification of systems, Stability of regulation, Quality of regulation, Feedback systems in Biology. SOLIDS AND FLUIDS: Properties of solids, Stress and strain in solids, Hook's law, stress-strain curves, Properties of fluids, Viscosity, Visco-elastic materials, fundaments of rheology. BIOPHYSICS OF TRANSPORT OF MATTER IN ORGANISMS: Diffusion, Fick´s law, Diffusion through membranes, Compartment systems, Flow of fluids, Stokes´s law, Hagen-Poiseuill´s law, Reynolds number. RADIOLOGY: Concepts and definitions, Detection and measurement of radiation, Sources of radiation, Radioisotopes in medicine and biology. OPTICS: Electromagnetic waves, nature of light, Interaction of light and biological systems, Biophysics of vision.

RECOMMENDED BOOKS 1. 2.

Bioelectronics: From Theory to Applications Edited by Itamar Willner and Eugenii Katz Wiley February 2005 CPL Press Biophysical Aspects of Transmembrane Signaling Edited by S Damjanovich Springer 2005. CPL Press

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT-205 IMMUNOLOGY – I 1. INTRODUCTION: Overview, Milestones in immunology, general immuno-biology, introduction to specific and non-specific immunity, and features of immune response. 2. CELLS AND ORGANS OF THE IMMUNE SYSTEM: lymphoid cells, heterogenecity of lymphoid cells, T cells, B cells, Null Cells, Monocytes, Polymorphs, primary and secondary lymphoid organs- thymus. Bursa of fabricus, spleen, lymph nodes, lymphatic system, mucosa associates lymphoid tissue (MALT), lymphocyte traffic. 3.HUMORAL IMMUNITY: Structure and function of antibody, structure and function of antigen, Antigen- Antibody reaction, affinity and avidity, high and low affinity antibody, immuno-globulins, classes and structure, molecular mechanism of generation of antibody, diversity, complement fixing antibodies and complement cascade. 4. CELL MEDIATED IMMUNITY: T-cell subsets and surface markers, T dependent and T independent antigen, recognition of antigens by T cells and role of MHC, class of MHC, structure of T cells antigen receptor, examples of cell mediated immunity. RECOMMENDED BOOKS 1. Immunology- Robin IM, Brostoff J and Male DK. 2. Principles of Cellular and molecular Immunology- Austyn JM and Wood KJ. 3. Immunology and Medical Microbiology -Singh RP 4. Introductory Immunology – Shetty, Nandini 5. Immuno-biotechnology – Yadav and Tyagi 6. Immuno-biotechnology- Naha and Narain. 7. Immunology –Singh and Bharat 8. Medical Immunology- StritesDP, Terr AL Opar TG.

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT- 207 TECHNIQUES IN BIOTECHNOLOGY-I 1. CULTIVATION OF MICROBES; Basic equipment (Incubators, sterilizers), Bacteriological techniques – culturing, sterilization, etc. Growth media and selection, Introduction to Industrial microorganisms. 2. Techniques and Types of Microscopy in Biotechnology: Introduction, brief History, Types of microscope and role in microbiology. Structure and function of microscope like Compound microscope, dissecting microscope, phase contrast microscope, Electron Microscope – Scanning electron microscopy, CT scan, Digital microscopy and their uses in biotechnology. 3. Spectroscopy: Introduction, UV/Visible and spectrophotoflourimeter, basic principles involved, different types of spectrometry – NMR, Magnetic resonance spectroscopy. Scope and use in biotechnology. Structural determination and analysis of biomolecules: Absorption spectroscopy and other optical techniques like fluorescence, infrared and Raman; 4

Chromatography: Introduction, principle, types – paper, two dimensional, thin layer, HPLC, ion-exchange chromatography etc. Uses, advantages and limitations.

5

Detection methods and Introduction to some emerging technologies: Brief Introduction to Hybridization and transfers techniques, PCR, Quantitative methods of nucleic acids, Electrophoresis, Radioisotope techniques, X-ray crystallography. pH and buffer concept. Microarrays, microwave technology, Genomics, proteomics, microfluidics, nanotechnology.

RECOMMENDED BOOKS 1. Biotechnology B D Singh, Kalyani Publication. 2. Introduction to Applied Biology and Biotechnology. K Vaidyanath, K Pratap Reddy, K Satya Prasad.

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT-209 BIOCHEMISTRY - II Section A 1.

Structure of functions of lipids, fatty acids, triacylglycerols, glycerophosholilpids, sphingomyelins, lipoproteins, Liposomes, biological membrances & micelles. Nucleic acids: Structure & properties of purine and pynrimidine bases. Nucleosides & nucleotides, biological functions of DNA and RNA species. Double helical model of DNA & forces responsible for it, short hand representation of nucleic acid back bone, denaturation od DNA, methods for isolation and purification of nucleic acids.

2.

Section B 3.

Introduction to enzymes & coenzymes, units of enzymes activity, enzyme nomenclature & classification, Enzyme Kinetics, effect of substrate concentration on Michaelis – Menten equation, determination of Km & its significance, effect of pH and temperature on rates of enzymes catalyzed reaction. Enzyme inhibitors & their importance, chemical methods of active site studies, Introduction to multisubstrate enzymes, allosteric enzymes & enzyme regulation, isoenzymes, enzyme immobilizationh.

4.

Books Recommended 1. 2. 3. 4.

rd

Principles of Biochemistry 3 edition, 200 : Lehninger, Nelson & Cox. th Biochemistry, 4 edition, 1995 : Luberts Stryer. W.H. Freeman and Company, New York. rd Text Book of Biochemistry, 3 Edition, 1986. K. Rangnathan Rao. Fundamentals of Biochemistry, 5th edition, 2000- J.L. Jain, Chand and Co., New Delhi.

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT - 211 ORGANIC CHEMISTRY LAB

1. Introduction to laboratory techniques through demonstration involving synthesis of selected organic compounds (e,g Aspirin, parabromoacetanilide, anthraquinone from anthracine, reduction of nitrobenzene.(any two) 2. Identification of organic compounds and derivatives (Any Ten) 3. Introduction to the use of stereo models BSBT- 213 Biochemistry-II LAB

1. 2. 3. 4. 5. 6. 7.

1. 2. 3. 4. 5. 6. 7. 8. 9.

Verification of Beer’s Law and Determination of Absorption Maxima Qualitative Estimation of Carbohydrates Quantitative Estimation of Carbohydrates Qualitative Estimation of Amino Acids Quantitative Estimation of Proteins Amino Acid and Carbohydrate Separation by Paper Chromatography Amino Acid and Carbohydrate Separation by Thin Layer Chromatography

BSBT- 215 IMMUNOLOGY – I LAB Haemagglutination assay Haemagglutination inhibition assay. Separation of serum from blood Separation of T and B cells from PBMC by nylon wool method. Isolation of mononuclear cells from peripheral blood and viability test by dye exclusion methods. Direct and indirect ELISA. Precipitation test Study of Lymph nodes in rats. Study of types of inflammation

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT-202 IMMUNOLOGY- II 1. Non-specific resistance (Non-specific immunity). Physical and chemical barrier, cellular barrier, inflammatory barriers, elevated body temperature. 2. Specific resistance (Acquired Immunity).Types of acquired immunity, immune response, neutralization, complex formation, opsonisation, complement system, antibody sources, serological reactions. 3

Techniques in Immunology. Precipitation test, Agglutination test, Fluorescent Antibody technique, Radioimmunoassay, Enzyme- linked immunosorbent assay (ELISA).

3. Perplexing Human Diseases. Autoimmunity, Immunodeficiency, Cancer. 4. Vaccines. Introduction, Ideal vaccine, conventional vaccine, live and dead vaccine, purified antigen vaccine, recombinant vaccine, live recombinant vaccine, DNA vaccine, Cultivation of viruses in vaccine production. Preparation of poliomyelitis vaccine, influenza vaccine, yellow fever vaccine, BCG vaccine, small pox vaccine, rabies vaccine. Storage of vaccines and potency tests. 5. Infection. Introduction, types and factors influencing infection.

RECOMMENDED BOOKS 1. 2. 3. 4. 5. 6.

Immunology- by Roitt and Roitt. th Immunology by Janis Kuby 5 edition. Principles of Cellular and molecular Immunology – Austyn JM and Wood Kj. Immunology and Medical Microbiology- Singh RP. Introductory Immunology- Shetty, Nandini Immuno- biotechnology- Yadav and Tyagi

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT 204 GENETICS 1. Introduction: Introduction to gene and protein, splice variants, secondary structure, triplet coding. 2

Sex determination. Dosage compensation, sex chromatin, chromosomal inheritance, Mitochondrial and chloroplast genetic system.

3

Gene expression: Gene organization and expression in prokaryotes and Eukaryotes.

4

Gene regulation. Prokaryotic and Eukaryotic gene regulation. Genetic control and development. Genetics of immunity. Transcription and translation in Eukaryotes. Protein structure and function. Chromosomal variation. Genetics of Cancer.

5

Population Genetics; Hardy-Weinberg equilibrium, evolutionary genetics

6

Analysis of mutation in biochemical pathway. One gene-one enzyme hypothesis. Isolation of auxotrophs, replica- plating technique.

7

Genomics. Introduction, genome sequencing projects, comparative genomic, gene prediction and counting. Genome evolution.

RECOMMENDED BOOKS 1. Current Perspectives in Genetics: Insights and Applications in Molecular, Classical, and Human Genetics, 2000 Edition by Shelly Cummings, Paperback: 170pages, Publisher: Books Cole. 2. Genes VIII by Benjamin Lewin. Publisher: Prentice Hall.

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT- 206 RECOMBINANT DNA TECHNOLOGY 1. Recombinant DNA technology: Gene cloning- steps in gene cloning, Restriction endonucleases- types, nomenclature, recognition sequence, cleavage pattern, frequency of recognition sites, modification of cut ends, isolation of desired gene 2 Vectors: Properties of good vector, cloning and expression vectors, E. coli vectors, Plasmids, PSC101, pBR322, pUC8, pGEM3Z and bacteriophage vectors. Cosmid vectors, Phagemid vector and Artificial chromosome vectors, shuttle vectors, yeast vectors,YAC, Vectors for plants and animals. cDNA Preparation, Isolation of mRNA, Genomic library, construction of genomic library. Integration of DNA insert into the vector: Integration of DNA inserts through site specific recombination, selection of desired recombinant DNA, Insertion of vector into the suitable host, , multiplication and expression of DNA insert in host genome, production of recombinant proteins in E. coli and other organism. 3 Chemical Synthesis of Genes and Polymerase Chain Reaction: Introduction, chemical synthesis of gene, gene amplification, procedure of PCR, primers, annealing temperature, Types of PCR- RT PCR, Inverse PCR, real time PCR, nested PCR. Analysis of PCR products, Applications of PCR and its limitations. 4

Genome maps: Introduction, definitions, genetic maps, genetic markers Application of molecular markers. Brief introduction to human genome project, automated chromosome-sorting, creation of contigs.

5

Understanding a gene function: Basic concepts of protein purification using affinity chromatography, SDS PAGE (polyacrylamide gel electrophoresis).

6

Gene Therapy. Introduction, Types of gene therapy, augmentation gene therapy and targeted gene therapy (general concept). RECOMMENDED BOOKS 1. Biotechnology B D Singh, Kalyani Publication. th 2. Gene cloning:T.A.Brown 4 edition Wiley publishers 3. Analysis of genes and Genomes:Richard Reece,John wiley &Sons 4. From Genes to Genomes:Jereny W dale and Malcolm von Schantz,John Wiley & sons. 5. Gene manipulations by Old and Primrose. 6. Molecular Cloning: A laboratory Manual by Maniatis Cold Spring Harbor Publications 7. Basic Biotechnology by Colin Ratlege (Editor), Bjorn Kristiansen, Cambridge University Press.

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT- 208 FUNDAMENTALS OF INDUSTRIAL BIOTECHNONOGY 1. Introduction: Industrially important microbes (E.Coli, Bacillus, Saccharomyces) 2. Role of Yeast in Industry: Transformation procedures, genetic markers for yeast transformation, industrial application. 3. Fermentation: Large scale fermentation: Design and operation of fermentors, Preparation of ideal growth medium for production of biomass and microbial products Fermentation reactions: Metabolic groups and pathways, culture preservation 4. Microbial Products: Microbial production of antibiotics, vitamins, organic acids 5. Industrial Biocatalyst: Introduction, scope and application, immobilization and its applications 6. Fermented Beverages: Beer, Whisky, Wine making and Vinegar making. 7. Role of industrial biotechnology : Introduction to fuel biotechnology, biofertilizers, biocontrol agents, scope and applications of environmental biotechnology and biosafety in industry. Books Recomended 1. Pelczar and E.C.S. (Jr.) Chan, 2000 : Microbiology, Tata McGraw Hill Pub. Co., New Delhi. 2. D.D. Bernard, R. Dulbecco, H.N. Eisen, and H.S. Ginsbery , 1990: Medical Microbiology, Harper and Row, New York. 3. MK.J. Waites et al ., 2001 : Industrial Microbiology, Blackwell Science Ltd., London. 4. Nicklin et al., 2001: Instant Notes in Microbiology, BIOS Scientific Publishers Ltd, U.K. 5. G.J. Tortora et al 1995: Microbiology : An introduction, Benjamin/Cummings Pub Co.,Inc.B.T. 6. Principles of fermentation Technology : By Stanbury and Whittakar, Orgamon Press 7. Industrial biotechnology by Cruger & Cruger , Tata McGraw Hill

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT-210 PLANT BIOLOGY 1. Introduction: Large scale cultures, agar cultures, suspension cultures, and problems in large scale cultures of plant cells. 2. Micropropagation: Proliferation of axillary buds, induction of adventitious buds, bulbs and protocorms, somatic embryo genesis, somaclonal variations, advantages and limitations. 3.

Virus Free Plants: Shoot meristem culture, cryotherapy, chemotherapy, virus indexing, and maintenance of virus free stock, applications and limitations.

4. Haploid Plants: Homozygous lines, gametoclonal variations, analytical breeding, production of all male populations, advantages and limitations. 5. Somaclonal variations: Isolation, molecular basis, induced mutations and somoclonal variations, advantages and limitations. 6. Embyo Rescue: Recovery of interspecific hybrids, propagation of orchids, shortening of breeding cycle. Overcoming dormancy. 7. Germplasm conservation: Freeze preservation, slow growth cultures, DNA clones, dessicated somatic embryos and artificial seeds. Advantages and limitations. Books : 1. J.Hammond, P. McGarvey and V.Yusibov (Eds) Plant Biotechnology Springer Verlag, 2000. 2. T-J Fu. G. Singh and W.R. Curtis (Eds) : Plant Cell and Tissue Culture for the production of Food Ingredients kluwer Academics/Plenum Press 1999. 3. H.S. Chawla : Biotechnology in crop Improvement . International Book Distributing Company, 1998 4. R.J. Henry; Practical Application of Plant Molecular Biology, Chapman and Hall, 1997. 5. P.K. Gupta: Elements of Biotechnology , Rastogi and C. Meerut , 1996 6 Bhojwani Rajdan: Plant tissue Culture: Theory and practice, a revised edition 7 Biotechnology B D Singh, Kalyani Publication

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PTU/BOS/BSBT/111/10-01-2008/batch-2006

BSBT-212 IMMUNOLOGY- II Lab ELISA test Precipitation test Study of lymph nodes in rats. Study and understanding of Bacterial, fungal, and viral infection in human by preparing models. 5. Study of types of inflammation. 1. 2. 3. 4.

BSBT 214 GENETICS Lab 1. Probability- Coin tossing and Color blindness. 2. Spotters related to theory: To show gene interaction, Rh factor- Erythroblastosis foetalis, Freemartin, Human abnormalities, Holandric genes (Hhypertrichosis), DNA modelwindow cutting of mutation and replication, Structure of Bacteriophage. 3. Model preparation related to theory.

BSBT- 216 RECOMBINANT DNA TECHNOLOGY Lab

1. 2. 3. 4. 5. 6.

Demonstration of purification of genomic DNA from Bacterial cells using chemical methods. Demonstration of purification of extra chromosomal DNA (plasmid DNA using chemical method). Purification of isolated DNA using a premade assay (kit). Demonstration of Agarose Gel Electrophoresis : preparing an agarose gel and visualizing both genomic and plasmid DNA. Demonstration of DNA digestion with restriction enzymes, agarose electrophoresis. Visualizing proteins- polyacrylamide gel electrophoresis

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