Research Scientist

RESUME Parashuram.Y.J. Biotechnology Division, Bejo Sheetal Seeds, Pvt Ltd. Bejo Sheetal Circle Mantha Road, Jalna (MS) 431 203 [email protected] [email protected] Total experience: 5+ years Research • Presently working as a Scientist at Biotechnology Division, Bejo Sheetal Seeds, Pvt Ltd. Jalna (MS). Work Profile 1. Production of Bt transgenic plants through Tissue Culture and In Planta Methodology (Cotton, Okra and Tomato) 2. Analysis of Transgenic (Putatives, T0 and T1) plants through PCR 3. Finding the Trouble Shooters in the transformation studies of Chilli, Tomato, Cauliflower and Okra 4. Monthly Documentation and reporting to the Principal Investigator 5. Green House Maintainance •

Worked as a PROJECT FELLOW for Ph.D under UGC sponsored project entitled “In-vitro production of Withanolides from cell and hairy root cultures of Withania somnifera Dunal”. Department of Botany Gulbarga University Gulbarga. (Synopsis accepted for the submission of Thesis).

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Completed M.Phil. and presented a dissertation entitled “ Callogenesis, Somatic embryogenesis and Regeneration Studies in Sorghum bicolor. Moench”. With First Class 76.2% in 2004, Department of Botany Gulbarga University Gulbarga.

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Worked as a Research Fellow at Lal Bagh Plant Tissue Culture Lab, Hulimavu Bangalore (October 1999- September 2000).

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Publications: 1. Sugarcane: In vitro, biotic and abiotic stresses and Molecular Biotechnology studies-A Review. F.T. Z. Jabeen., Y.J. Parashuram and Srinath Rao*. Plant cell Biotechnology & Molecular Biology. Volume. 10 (1 & 2). 2009. 2. Somatic Embryobenesis and Plant Regeneration of Commercially Important and Endangered Banana (Musa Acuminata Colla) CV. Red Banana. C. Nagappa, Y. J. Parashuram and Srinath Rao. International Journal of Biotechnology & Biochemistry. Volume 5, Number 3. Pp. 261-269. 2009. 3. In vitro plant regeneration from cotyledonary leaf callus in Withania somnifera (Dunal). An important medicinal plant. Y.J. Parashuram, C. Nagappa and Srinath Rao. International Journal of Biotechnology & Biochemistry. Volume 5, Number 2 (2009) pp. 179-188. 4. High root biomass production in auxin-supplemented cultures of Withania somnifera (Dunal). Y.J. Parashuram and Srinath Rao. Plant Cell Biotechnology and Molecular Biology Journal. An International Journal on Biotechnological Research. Volume. 9 (3 & 4). 2008. 5. “In vitro clonal propagation of commercially important and endangered Banana (Musa accuminata colla.) cv. Red Banana”. Nagappa C, Kaviraj C P, Y.J. Parashuram, Kaveri K and Srinath Rao. Vol 3 (3): pp 327-332. 2008. 6. High frequency plant regeneration from shoot tip culture of Chilli (Capsicum annuum L.). Srinath Rao, G.S Pratibha, Y.J. Parashuram and C.P. Kaviraj Plant cell Biotechnology & Molecular Biology. Volume 7 (3&4): 163-166. 2006. 7. Antimicrobial activity of callus and natural plant extracts of Abrus precatorius (L)”-An important Medicinal plant. C.P.Kaviraj, N.C. Siddaling, R.B. Venugopal, S.G.Kiran, F.T.Z. Jabeen, Y.J. Parashuram and Srinath Rao. Asian Jr. of Microbiol. Biotech. Env. Sc. Vol. 7, No. (3): 453-456. 2005.

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Teaching • •

Worked as a Guest Lecturer in Botany for Degree College under UGC programme for the period of 10 months in Karnataka College Bidar. Karnataka (December 2000- October 2001). Worked as a Lecturer in Sericulture for Vocational Course in Sericulture Government College Gulbarga, Karnataka. (July 2002- October 2003).

Conferences Attended: 1. National Symposium on Biotechnology for a Better Future, 15-17 January 2004, held at St Alosys College, Mangalore. Contributed a poster presentation entitled “Comparative Antimicrobial activity of callus and natural plant extracts of Abrus precatorius (L)”. 2. National Symposium on Plant Biotechnology: New Frontiers, 18-20 November, 2005, held at Central Institute of Medicinal and Aromatic Plants, Lucknow. Contributed a poster presentation entitled “Somatic embryogenesis in Jawar (Sorghum bicolor L.) Moench”. 3. International Symposium on Frontiers in Genetics and BiotechnologyRetrospect and Prospect, 8-10 January 2006, held at Department of Genetics Osmania University, Hyderabad. Contributed a poster presentation entitled “ Hairy roots” 4. National Symposium on Biotechnology “ In vitro Regeneration and Cloning Technique”, 12-14 October 2007, held at The Forest Research Institute, Dehradun (U.A). Contributed a poster presentation entitled “Direct Rhizogenesis from Cotyledonary Explants of Withania somnifera Dunal An Important Medicinal Plant”. 5. “Awareness Training programme on Biodiversity Related Issues and Peoples biodiversity Register” at Department of Biotechnology, Gulbarga University, Gulbarga on21st Sep 2007. 6. National Seminar on “Currents trends in Biotechnology” at Department of Biotechnology, Lal Bahadur College, Warangal from 30th November to 1st December 2007. 7. National Symposium on “Plant Biotechnology for Conservation, Characterization and Crop Improvement” And 29th Annual Meeting of Plant Tissue Culture Association (India), from February 8-10, 2008 held at Department of Biotechnology, Mohanlal Sukhadia University, Udaipur. (Rajasthan.)

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8. International Conference on Plant Biotechnology and Molecular Biology: 15-17 August 2008. Organized by Department of Biotechnology Kakatiya University, Warangal- 506 009. A.P. India. Workshops/Training Programmes attended 1. Fifth all India summer research training programme on molecular Techniques, May 16- 30, 2007(15 days) sponsored by Tamil Nadu State Council for Science and Technology, Chennai; NCSTC, New Delhi, Sengunthar Education Trust, Organized by SMART, PG and Research Department of Microbiology and Biotechnology Tiruchengode, T.N. 2

“National workshop on Challenging Techniques in Phytochemical, Analytical & Pharmacological Evaluation of Herbal Products” From 11-12th January 2008. Organized by Luqman College of pharmacy, Gulbarga.

Educational Qualification •

B.Sc. with the combination of Botany, Zoology and Sericulture from the Gulbarga University Gulbarga with First Class (61.4%) in 1997.

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M.Sc. in Botany, Specialization in Plant tissue Culture and Genetic Engineering from the Gulbarga University Gulbarga with First Class (64.1%) in 1999.

Computer Knowledge •

MS-Office (MS-world, MS-Excel, MS- Power Point)

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DTP (Desk Top Publishing)

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Good Knowledge of Internet Access

Personal Profile: Name: Father’s Name Date of Birth Sex Marital Status Nationality E-mail

Parashuram.Y.J. Yallappa. J. 09 June 1976 Male Single Indian [email protected]

A SYNOPSIS OF THE RESEARCH PROJECT CARRIED  Optimized the media components and hormonal concentrations for the growth of callus and cell cultures of Withania somnifera and Withaferin–A 4

accumulation. (Standardization of media components, use of different growth regulators, collection of callus of different days, HPLC analysis for Withaferin–A).  A single clone has been developed and stable production of Withaferin–A in long term culture by plating the cells. (Cell suspension culture of the callus and HPLC analysis of Withaferin–A).  The ability of the in vitro raised shoots; roots and callus were assessed to synthesize the Withaferin–A. (Effect of the individual growth regulator on different explants and their effect on production Withaferin–A).  Initiated the Hairy Roots using two strains of Agrobacterium rhizogenes (LBA 4404 and LB 9402) and accumulation of Withaferin–A was studied. (Co cultivation of different explants with Agrobacterium rhizogenes, induction of hairy roots (Transformation), GUS expression, PCR and HPLC analysis for Withaferin–A).  Enhancement of Withaferin–A using biotic elicitors in callus cultures of Withania somnifera. (Use of biotic elicitors prepared from bacterial and fungal cultures in standardized media for the enhancement of Withaferin–A).  Extraction and HPLC analysis of Withaferin–A was carried using different solvents.

TECHNIQUES KNOWN  Plant Micro propagation Techniques.  Plant Biotechnology techniques viz., • • • • • •

Isolation of DNA Isolation of Plasmid Gene Transformation through Co-Cultivation and Particle Bombardment GUS expression in transformed tissues PCR HPLC

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“In vitro production of withanolides from cell and hairy root cultures of Withania somnifera (L) Dunal”. Introduction: Tissue is an important area of Biotechnology can be used to improve the productivity of planting material through enhanced availability of identified planting stock with desired traits. A German scientist Haberlandt put forth the idea of cell and tissue culture in 1902. All attempts to culture the plant cells and tissue culture were successful till the 1930. Around 1939 White, Nobecourt and Gautheret independently reported the possibility of culturing plant tissues for definite periods. Micro-propagation is one of the important contributions of Plant Tissue Culture to Medicinal plant propagation and has vast significance. The name micro-propagation derives from the miniature shoots/plantlets initially produced from this method of plant propagation. This technique provides a rapid reliable system for a production of large number of genetically uniform plantlets. The primary goal of the tissue culture research is crop improvement. Tissue culture techniques after advantages over the conventional methods of plan breeding principally because new plant traits can be selected at the culture level in the laboratory instead of the whole plant level in the field. This affords advantages of time in that cellular screening for traits requires weeks or months where as screening of whole plants typically requires entire growing season. In recent time plant tissue culture in conjunction with Genetic engineering and related techniques, is a promising and potentially emerging area of plant biotechnology and has generated great interest and speculation for genetic manipulation of crop plants with desirable results. The plant tissue culture was exploited both for basic and applied aspects of plant research encompassing haploidy, mutagenesis, somatic embryogenesis, somaclonal variations, protoplast fusion and genetic manipulation and even commercial exploitation. Plant description: Withania somnifera (The Indian Ginseng) belongs to family Solanaceae includes 42 species and a common plant native of India. The plant is small subtropical medicinal plant with anti carcinogenic and adaptogenic properties for which withanaloids and

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sitoinlosides are responsible. It is a middle sized erect shrub, growing up to 1.5 meters tall. It has egg-shaped, hairy leaves up to 10 cms, small, pale green flowers in clusters of about 25 and smooth; spherical, red fruits with yellow seeds. Material and Methods: •

Seeds were collected from the Agriculture Research Station Gulbarga, seeds were germinated in vitro and different parts of the plants such as shoot tips, cotyledons, shoots and leaves were used as explants.

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Callus induced from different explants such as shoots, leaf and root using different concentrations of auxins viz., 2,4-D, 2,4,5-T, NAA and PAA alone or in combination with BAP and Kn cytokinins.

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In an optimized media and explant, accumulation of withaferin-A was measured. Development of single clones for high and stable production of secondary metabolites (withaferin-A) in long-term culture by plating the cells.

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Multiple shoots were induced by using different explant viz., stem, leaf and callus by using different growth regulators viz., BAP, TDZ and Zeatin alone or in combination with IBA, Kn and IAA.

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Differentiate shoots, roots from callus culture and to asses their ability to synthesize withaferin-A.

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In vitro regenerated shoots were transferred to rooting medium containing different concentrations of NAA, IBA and PAA.

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Genetic transformation was achieved using two strains of Agrobacterium rhizogenes.

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Initiation of hairy root culture using Agrobacterium rhizogenes and to know their ability for the production of withaferin-A.

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The effect of various biotic elicitors (prepared from bacterial and fungal culture) was carried if they can enhance withaferin-A accumulation.

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Co–cultivated tissue were examined for genetic transformation by GUS analysis and PCR amplification.

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To immobilize the cells using polymers and to find out their role on withaferin accumulation in cell culture.

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The production of withaferin-A was carried in a large-scale bioreactor using free and immobilized cell culture.

Principal Investigator Prof. Srinath Rao Plant Tissue Culture and Genetic Engineering Lab Department of Botany Gulbarga University, Gulbarga-585106 Karnataka- INDIA [email protected] +919986110109

References: 1.

Dr.P.B.Kavikishore Professor and Head Department of Genetics. Osmania University, Hyderabad – INDIA [email protected] +919948489567

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Dr. S.S Udikeri Agriculture Research Station Dharwad Farm (Hebballi Farm) Navalgund Road Dharwad – 7 [email protected] +919448136821

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