R05222302-instrumental-methods-of-analysis

Set No. 1

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 INSTRUMENTAL METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. (a) Define the terms qualitative & quantitative analysis. (b) Differentiate between micro & semi micro analytical methods. (c) What are advantages & limitations of chemical methods.

[6+6+4]

2. Compare & contrast the techniques phase -contrast microscopy & differential interference contrast microscopy (DIC) [16] 3. Explain the following terms: (a) Gravitational force. (b) Centrifugal force (c) Sedimentation coefficient.

[5+5+6]

4. You have two samples of DNA derived from different organisms,A & B. DNA from A shows an increase in optical density begnning at 700 C & plateau at 800 C. DNA from B shows a corresponding increase in O.D beginning at 650 C & plateau out at 760 C. What is your conclusion. Justify your answer. [16] 5. (a) Define fluorescence. Derive an expression relating intensity of fluorescence & concentration. (b) Write a brief note on : i. Triplet state ii. Doublet state iii. Singlet state.

[8 × 2 = 16]

6. (a) Discuss the basic principle of infra red spectroscopy? (b) Briefly describe the scanning of infrared spectrum of an organic compound. [8 × 2 = 16] 7. Explain the following : (a) Free inductive decay (b) Hetero nuclear decoupling (c) Off resonance coupling (d) Chemical shift.

[8 × 2 = 16]

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Set No. 1

Code No: R05222302

8. Give an account on electronic structure & hyperfine splitting in ESR spectroscopy? [16] ⋆⋆⋆⋆⋆

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Set No. 2

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 INSTRUMENTAL METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Give a brief account on methods for reporting analytical data?

[16]

2. Compare & Contrast the optical magnification obtained by the bright & dark field microscopy along with their specific applications. [16] 3. (a) Give the basic principle involved in analytical centrifugation. (b) What are the important application of this technique in biochemistry? [8 × 2 = 16] 4. You have two samples of DNA derived from different organisms,A & B. DNA from A shows an increase in optical density begnning at 700 C & plateau at 800 C. DNA from B shows a corresponding increase in O.D beginning at 650 C & plateau out at 760 C. What is your conclusion. Justify your answer. [16] 5. (a) Define optical activity? What is the principle involved in circular dichroism spectroscopy? (b) Differentiate the polarimetry & circular dichroism.

[8 × 2 = 16]

6. Discuss the principle, importance & applications of mass spectrometry?

[16]

7. (a) Give the principle underlaying in NMR spectrometry? (b) Describe the component of a NMR spectrometer with a neat labeled diagram. [8 × 2 = 16] 8. (a) What is the principle of ESR. Discuss some important applications of ESR. (b) What are the limitations of ESR. ⋆⋆⋆⋆⋆

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[8 × 2 = 16]

Set No. 3

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 INSTRUMENTAL METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Give an account of the instruments involved in electrochemical methods of analysis along with their principle of working. [16] 2. (a) Give the basic principle involved in the viewing an object in dark field. (b) Draw a neat labeled diagram of optical pathway of dark field microscope. (c) Give its specific use.

[5+6+5]

3. (a) Give the basic principle involved in analytical centrifugation. (b) What are the important application of this technique in biochemistry? [8 × 2 = 16] 4. What are the different regions of the electromagnetic spectrum & where are they used for? [16] 5. Define the following terms: (a) chiral compounds (b) cotton effect (c) circular birefringence.

[6+5+5]

6. How will you distinguish between intermolecular & intramolecular hydrogen bonding by IR spectroscopy? [16] 7. (a) Explain the principle involved in CMR spectra? (b) Why it is not possible to determine relative ratio of carbon atoms in a compound by integration of peak areas in 13C NMR as in PMR?. [8 × 2 = 16] 8. Why ESR spectroscopy is widely used in the study of chemical, photochemical & electrochemical reactions? Justify your answer. [16] ⋆⋆⋆⋆⋆

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Set No. 4

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008 INSTRUMENTAL METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. Write an account on analytical methods based on the physical property measurement? [16] 2. Narrate the principle of operation of a fluorescence micrscope. Give its applications in biology? [5+11] 3. Calculate the molecular mass of the protein where the sedimentation coefficient of the protein is 7.75 ×10−13 s. In subsequent analysis the protein was found to have an average diffusion coefficient 0f 4.0 × 10−11 m2 s−1 & the a partial specific volume of 0. 734 ×10−3 m3 kg −1 . (a) convert the molecular mass to relative molecular mass. (b) Express the molecular mass in Daltons.

[8 × 2 = 16]

4. Define the terms: (a)

i. ii. iii. iv.

Radiant power( P ) Path length of radiation absorptivity molar absorptivity.

(b) Describe & derive the Beer’s law. (c) What are its limitations & deviations.

[4+6+6]

5. (a) What is atomic absorption. What are its advantages & disadvantages. (b) Give some of its limitations.

[8 × 2 = 16]

6. (a) What are the different regions of infrared radiation? (b) Explain various types of stretching & bending vibrations with suitable examples. [8 × 2 = 16] 7. (a) Why does a signal for a particular set of protons is split into a multiplet. (b) explain with suitable examples the trem spin-spin coupling

[8 × 2 = 16]

8. (a) Which of the following two formulas is correct for the complex obtained between copper ion & 8 quinolinethanol (C9 H7 N5 ) i. Cu(I) (C9 H7 N5 )(C9 H6 N5 ) ii. Cu(II) (C9 H6 N5 )2 1 of 2

Set No. 4

Code No: R05222302

show that the ESR approach is appropriate for this determination. (b) Which is one of the best known free radicals used in calibrating ESR spectra? [8 × 2=16] ⋆⋆⋆⋆⋆

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