Plasma Proteins

Plasma Proteins Dr. Niranjan Murthy H L Asst Prof of Physiology SSMC, Tumkur

• Albumin • Globulin- α₁, α₂, β₁, β₂, γ • Fibrinogen Origin: Liver- Albumin & Fibrinogen Plasma cells, lymphocytes, tissue macrophages- Globulin

S. Albumin: 3.5-5g/dL S. Globulin: 2-3g/dL S. Fibrinogen: 0.3g/dL

Other proteins • • • • • • •

Antithrombin-III Haptoglobulin Transferrin Angiotensinogen Apolipoprotein B Clotting factors Antithrombin C

Separation Techniques 1. 2. 3. 4. 5.

Salt precipitation Electrophoresis Cohn’s fractionation Ultracentrifugation Gel filtration

ELECTROPHORESIS

Whipple’s Experiment • Relation of diet to plasma • Plasmapheresis 3 5 6 7

types of cellular proteins: Fixed or Indispensible cell proteins Dispensable reserve proteins Labile reserve proteins

Functions of plasma proteins 1. 2. 3. 4. 5. 6. 7.

Coagulation of Blood Maintain Colloid osmotic pressure Acid-Base balance Immunity Transport functions Maintenance of viscosity of blood Reservoir function

Variations in plasma protein levels Reduced albumin: 2 Infancy 3 Pregnancy 4 Hepatitis 5 Cirrhosis 6 Nephrosis 7 burns

Increased globulin: 2 Cirrhosis of liver 3 Tuberculosis 4 Lymphatic leukemia Decreased Fibrinogen: 6 Congenital 7 DIC Increased Fibrinogen: 9 Pregnancy 10 malaria