Perhitungan Semsol Gel Tambahan.docx

Perhitungan Baku dengan persamaan y = 0,0254x + 0,0188, maka mendapatkan hasil sbb: √t 2,236 3,162 3,872 5,477 7,745 9,486 10,954 12,247

Waktu 5 10 15 30 60 90 120 150

Abs referen 0,174 0,178 0,118 0,216 0,285 0,335 0,367 0,445

Kadar referen 6,110 6,268 6,661 7,746 10,480 12,449 13,709 16,780

Abs sampel 0,068 0,079 0,087 0,124 0,187 0,236 0,288 0,321

Kadar sampel 137,084 169,073 193,064 317,602 476,504 617,764 768,648 868,082

Perhitungan Fluks Uji Pelepasan Gel Na Diklofenak (referen) Waktu

√t

Kadar (C)

Kadar kumulatif (ppm)

5 10 15 30 60 90 120 150

2,236 3,162 3,872 5,477 7,745 9,486 10,954 12,247

6,110 6,268 6,661 7,746 10,480 12,449 13,709 16,780

6,110 12,378 19,039 26,785 37,265 49,714 63,423 80,203

Perhitungan: 

Cn5

5

= 6,110 + 500 . 6,110 = 6,171 ppm



Cn10

5

= 6,268 + 500 . 6,268 = 6,330 ppm



Cn15

5

= 6,661 + 500 . 6,661 = 6,727 ppm



Cn30

5

= 7,764 + 500 . 7,764 = 7,841 ppm



Cn60

5

= 10,480 + 500 . 10,480 = 10,584 ppm



Cn90

5

= 12,449 + 500 . 12,449 = 12,573 ppm



Cn120

5

= 13,709 + 500 . 13,709 = 13,846 ppm



Cn150

5

= 16,780 + 500 . 16,780

Kadar Koreksi wurster (Cw) 0 0,061 0,123 0,190 0,267 0,372 0,497 0,634

Kadar sebenarnya (C+Cw)*500

Jumlah Kumulatif

%pelepasan (%b/b)

3055 3164,5 3392 3968 5373,5 6410,5 7103 8791

436,73 447,98 476,07 554,91 749,04 889,80 979,90 1199,36

2,183 2,239 2,380 2,774 3,745 4,445 4,890 5,996

= 16,947 ppm Penentuan bahan aktif yang terlepas dari basis (Jumlah Kumulatif) Luas permukaan kulit  C = 3,14 x (1,5 cm) 2 = 7,065 cm2 

X5

=



X10

=



X15

=



X30

=



X60

=



X90

=



X120

=



X150

=

6,171 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 6,330 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 6,727 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 7,841 𝑝𝑝𝑚 𝑥 500 𝑚𝑙

= 436,73 μg/cm2 = 447,98 μg/cm2 = 476,07 μg/cm2 = 554,91 μg/cm2

7,065 𝑐𝑚2 10,584 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 12,573 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 13,846 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 16,947 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2

= 749,04 μg/cm2 = 889,80 μg/cm2 = 979,90 μg/cm2 = 1199,36 μg/cm2

Kadar Koreksi Wurster (Cw) 5𝑚𝑙



Cw5



Cw10 = 500𝑚𝑙 x 6,110 ppm = 0,611 ppm



Cw15 =



Cw30 = 500𝑚𝑙 x 19,039 ppm = 0,190 ppm



Cw60 = 500𝑚𝑙 x 26,785 ppm = 0,267 ppm



Cw90 = 500𝑚𝑙 x 37,265 ppm = 0,372 ppm



Cw120 = 500𝑚𝑙 x 49,714 ppm = 0,497 ppm



Cw150 = 500𝑚𝑙 x 63,423 ppm = 0,634 ppm

= 500𝑚𝑙 x 0 ppm = 0 ppm 5𝑚𝑙 5𝑚𝑙 500𝑚𝑙 5𝑚𝑙

x 12,378 ppm = 0,123 ppm

5𝑚𝑙 5𝑚𝑙 5𝑚𝑙 5𝑚𝑙

% pelepasan Bobot referen = 2,000 gram 1

1% dari bobot = 100 x 2 gram = 20000 μg 

%b/b5 =



%b/b10 =



%b/b15 =



%b/b30 =



%b/b60 =

436,73 20000 447,98 20000 476,07 20000 554,91 20000 749,04 20000

x 100% = 2,183 % x 100% = 2,239 % x 100% = 2,380 % x 100% = 2,774 % x 100% = 3,745 %

889,80



%b/b90 =



%b/b120= 20000 x 100% = 4,890 %



%b/b150=

20000 979,90

x 100% = 4,445 %

1199,36 20000

x 100% = 5,996 %

Persamaan regresi antara √t dan jumlah kumulatif iki aku sek bingung r= y=



Perhitungan Fluks Uji Pelepasan Gel Na Diklofenak (sampel)

Waktu

√t

Kadar (C)

Kadar kumulatif (ppm)

5 10 15 30 60 90 120 150

2,236 3,162 3,872 5,477 7,745 9,486 10,954 12,247

1,937 2,370 2,685 4,142 6,622 8,551 10,598 11,897

1,937 4,307 6,992 11,134 17,756 26,307 36,905 48,802

Perhitungan: 

Cn5

5

= 1,937 + 500 . 1,937 = 1,956 ppm



Cn10

5

= 2,370 + 500 . 2,370 = 2,390 ppm



Cn15

5

= 2,685 + 500 . 2,685 = 2,711 ppm



Cn30

= 4,142 +

5 500

. 4,142

= 4,183 ppm 

Cn60

5

= 6,622 + 500 . 6,622 = 6,688 ppm



Cn90

5

= 8,551 + 500 . 8,551 = 8,636 ppm



Cn120

5

= 10,598 + 500 . 10,598 = 10,703 ppm

Kadar Koreksi wurster (Cw) 0 0,019 0,043 0,070 0,111 0,178 0,263 0,369

Kadar sebenarnya (C+Cw)*500

Jumlah Kumulatif

%pelepasan (%b/b)

968,5 1194,5 1364 2241 3366,5 4364,5 5430,5 6133

137,084 169,073 193,064 317,602 476,504 617,764 768,684 868,082

0,684 0,847 0,963 1,585 2,378 3,083 3,836 4,332



Cn150

5

= 11,897 + 500 . 11,897 = 12,015 ppm

Penentuan bahan aktif yang terlepas dari basis (Jumlah Kumulatif) Luas permukaan kulit  C = 3,14 x (1,5 cm) 2 = 7,065 cm2 

X5

=



X10

=



X15

=



X30

=



X60

=



X90

=



X120

=



X150

=

1,956 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 2,390 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 2,711 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 4,183 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 6,688 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 8,636 𝑝𝑝𝑚 𝑥 500 𝑚𝑙

= 138,4 μg/cm2 = 169,1 μg/cm2 = 191,9 μg/cm2 = 296 μg/cm2 = 473,3 μg/cm2 = 611,2 μg/cm2

7,065 𝑐𝑚2 10,703 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2 12,015 𝑝𝑝𝑚 𝑥 500 𝑚𝑙 7,065 𝑐𝑚2

= 757,5 μg/cm2 = 850,3 μg/cm2

Kadar Koreksi Wurster (Cw) 5𝑚𝑙



Cw5



Cw10 = 500𝑚𝑙 x 1,9 ppm = 0,019 ppm



Cw15 = 500𝑚𝑙 x 4,3 ppm = 0,043 ppm



Cw30 = 500𝑚𝑙 x 7 ppm = 0,070 ppm



Cw60 = 500𝑚𝑙 x 11,1 ppm = 0,111 ppm



Cw90 = 500𝑚𝑙 x 17,8 ppm = 0,178 ppm



Cw120 = 500𝑚𝑙 x 26,3 ppm = 0,263 ppm



Cw150 = 500𝑚𝑙 x 36,9 ppm = 0,369 ppm

=

500𝑚𝑙 5𝑚𝑙

x 0 ppm = 0 ppm

5𝑚𝑙 5𝑚𝑙 5𝑚𝑙 5𝑚𝑙 5𝑚𝑙 5𝑚𝑙

% pelepasan Bobot referen = 2,004 gram 1

1% dari bobot = 100 x 2,004 gram = 0,02004 g = 20040 μg 

%b/b5 =



%b/b10 =



%b/b15 =

137,084 20040 169,073 20040 193,064 20040

x 100% = 0,684 % x 100% = 0,847 % x 100% = 0,963 %



%b/b30 =



%b/b60 =



%b/b90 =



%b/b120=



%b/b150=

317,602 20040 476,504 20040 617,764 20040 768,648 20040 868,082 20040

x 100% = 1,585 % x 100% = 2,378 % x 100% = 3,083 % x 100% = 3,836 % x 100% = 4,332 %

Persamaan regresi antara √t dan jumlah kumulatif r = 0,9746 y = 4,347 x + 10,716