Micro Gas Monitoring, Peter, Aniediabasi John; Antai, Ekpo Eyo (phd)

VOLUMETRIC ASSESSMENT OF LAB-BASED GAS PRODUCED FROM CONTROLLED WASTE COMPONENTS *Peter A. John & **Ekpo E. Antai +234-803-9693897; [email protected]

EXPERIMENTAL DESIGN Sampling Design Waste material made up of 100% organics were collected, segregated and identified. The waste components includes market waste (A), organic domestic waste (B), and animal droppings (C), wood chippings/sawdust (D) was added using Controlled Waste Mix ing Method (CWMM). For the in-situ corrosion exercise, special in-situ/ bench top set-up was prepared using diluted, sterilized, synthesized sea habitat water in a microcosm. This was subjected to ambient laboratory conditions. MATERIALS Gasometric apparatus Waste specimen Identified species isolated and purified from the specimen. THEORY Gas production can be improved by an equal improvement in some environmental factors. Control set-up was also included. Waste conversion: Microbe-Mediated Catabolic Process

Figure 1: Volumetric Assembly The apparatus illustrated above is an assembly made up of four (4) pieces, namely; double neck round bottom flask , flexible connecting cord, condenser with calibrated burette and funnel. All these are held in place by retort stands. This set-up is a closed system, which does not allow the escape of matter (gas). It has a major advantage of being used as an apparatus for both batch and continuous processes. Gas release during catabolic processes can be indicated by upward displacement of the paraffin liquid contained in the U-region of the flexible cord. Upward displacement of meniscus is compared with the initial level of the meniscus to obs erve changes. This change in level is recorded from the values on the calibrated burette in the condenser and can be expressed as;

Gas volume (ΔL) = L 2 – L 1; where L1 and L2 is the initial and final level of meniscus respectively.