Medirox Presentation Plb

MEDIROX VISCOSITAS

Research and Development • • • •

Prof. Tomas Lindahl Dr. Steffen Rosén Dr. Mats Rånby Prof. Leif Bohlin

Scientific adviser Reagent development Reagent development Rheology

Patent Free Oscillation Rheometry (FOR)

• Viscosity = (f) log damping (mPas, cSt) • Elasticity = (f) frequency change 2 (Pa) • Shear rate = 2π frequency = 65/sec

Key features FOR • Does not destroy fibrin fibres. (Increased sensitivity) • Good for measurements of long clotting times. • Viscosity, elasticity, clotting time in real time measurement and in SI units. • Oscillation keeps sample mixed. • Disposable measuring chambers / cups.

Plasma Viscosity Clinical interpretation Plasma viscosity The changes in plasma viscosity reflect the changes in the concentration of one or more of the plasma proteins. The influence is bigger on large protein fraction and if the protein molecule departs from a sphere.

Physiology (PV) • • • • • • • •

Sex Under 3 years Diet Time of day Rest Mensturation Pregnancy Day to day

Same range 0,25 mPa.s lower No effect No effect +/- 0,03 No effect Raisis up to 1,8 mPa.s 0,05 mPa.s can be of interest

PV – ESR Plasma Visc

ESR

Plasma can be analyzed up to one week after collection

Test must be started within 4 hours after collection. Exception: Samples with anti-coagulant Na2EDTA can be analyzed up to 24 hours.

Anemia need not be considered

There is no agreement how anemia should be considered.

Variation in red cells surface has no effect

Red cell surface differences affect sedimentation time.

Results in 30 seconds

Results in minimum 30 min.

PV-ESR • • • •

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Plasma Visc Narrow normal range. No falsh negatives. No neg. effect from high Steroides, Salicylates. Myeloma and macroglobulinaemia are characteristic. Sensitive, specific, good reproducibility

ESR Wide and differs Falsh negatives happens Decreases Not destinguished from other diseases. Moderat/Low

Measuring cups.

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PV Viscosity (room temp). POM cup. Clotting time ReoRox. POM or C-cup. Initial Rate. POM or C-cup. End point Elasticity. Gold bob/cup. Fibrionolysis. Gold cup.