Kp Abstract 2

EVALUATION OF ANTI ULCER ACTIVITY OF AYURVEDIC FORMULATION-(Sooktyn) BY USING ASPIRIN PLUS PYLORUS LIGATED MODEL Santosh* Care college of pharmacy, Ogulapur, Warangal Raghuram.A, Krishna Prasad.D, Elango.K Department of Pharmacology, J.S.S. College of Pharmacy, Ooty-643001

ABSTRACT

Sooktyn bhasma is unique herbal preparation of Ayurvedic system of Indian traditional medicine. It is used traditionally for antiulcer. Sooktyn was evaluated for its antiulcer activity in experimental animals at dose levels of 350 and 700mg/kg. The experimental paradigms used for antiulcer activity were aspirin plus pylorus legated model in rats. It was observed that 700mg/kg dose of Sooktyn bhasma produced significant (P<0.01) reduction in gastric volume, free acidity, total acidity, ulcer index and sodium concentration and also shown significant (P<0.01) increase in the potassium when compared when compared to vehicle control animals. These findings suggest that the test drug shows anti-ulcer activity either by reducing the gastric volume or by mucous secretion in the stomach. Key words: Ulcer, herbal preparation, pylorus ligated.

Objective: To study the antiulcer activity Sooktyn bhasma on aspirin plus pylorus legated model in rats. Method: Aspirin induced ulcerogenesis in pylorus ligated model (Anoop.A et., al 2003): Adult Wistar albino rats of either sex weighing 180-250 g were divided into 4 groups of six animals each and placed in cages with grating floor to avoid coprophagy and fasted for 48 hours allowing free access of water. Group 1: Served as solvent control (0.3% CMC) Group 2: Received Omeprazole (2 mg/kg) Group 3: Received Sooktyn (350mg/kg) Group 4: Received Sooktyn (700mg/kg) In aspirin-induced ulcerogenesis in pylorus ligated rats, aspirin is administered at a dose of 200 mg/kg orally in a suspension prepared in 0.3% CMC, 1h prior to pyloric ligation (time interval between reference drugs and aspirin should be 1 h). The test drugs were administered twice daily for 2 days and standard drugs were administered once daily orally for 2 days prior to and one hour before to aspirin administration. Under Ketamine and Xylazine anaesthesia, (Jim Gourdon 2002) the abdomen was opened by a small midline incision below the xiphoid process. Pyloric portion of the stomach was slightly lifted out and ligated avoiding traction to the pylorus or damage to its blood supply. The stomach was replaced carefully and the abdominal wall closed by uninterrupted sutures. The animals were deprived of both food and water during the post-operative period. Four hours after the ligation the animals were sacrificed using excess ether anaesthesia. The stomach was excised carefully keeping the oesophagus closed, opened along the greater curvature and collect the gastric juice, centrifuged and then subjected to various biochemical analysis. The mucosa was flushed with saline and the stomach pinned on a frog board.

The Ulcer index was calculated according to the method of (Anoop.A et., al 2003) The lesions were counted with the aid of hand lens (10X) and each given a severity rating as follows Ulcer Score 0 1 2 3 4

Descriptive Observation Normal Scattered haemorrhagic spots Deeper haemorrhagic spots and some ulcers Haemorrhagic spots and ulcers Perforation

The ulcer score was divided by a factor of 10 to get the ulcer index. % Ulcer protection was calculated according to the standard formula by Njar et al., (1994) Ulcer Index in Control – Ulcer index in Test --------------------------------------------------------- X 100 Ulcer Index in Control The ulcer area was calculated by using the formula, Ulcer area=length x width of ulcer (mm)2 The gastric juice that is collected was centrifuged. The volume and pH was recorded and subjected to bio-chemical estimations like free acidity, total acidity, sodium and potassium of the gastric juice were calculated. Results: Sooktyn bhasma at 700mg/kg dose level shows significant (P<0.01) reduction in gastric volume, free acidity, total acidity, ulcer index and sodium concentration and also shown significant (P<0.01) increase in the potassium when compared to vehicle control animals. The anti-ulcer activity of test drug at 700mg/kg dose levels shows equipotent to that of Omeprazole. Conclusion: Our results suggest that Sooktyn bhasma possesses significant antiulcer property which could be either due to cytoprotective action of the drug or by strengthening of gastric mucosa and thus enhancing mucosal defense.

Bibliography:

Anoop A and Jegadeesan M. Biochemical studies on the anti-ulcerogenic potential of Hemidesmus indicus R.Br. var. indicus. Journal of Ethnopharmacology. 2003, 84: 149156. Jim Gourdon. Rodent anesthesia. (Online) Available from URL: http: // www.conell university.org (Accessed 2008, January 17).