Journal Article Assignment

Journal Article Assignment: Endocytosis

Name: Lisa Liu ID: 20267802 Section: 121 TA: Stacey

1. Endocytosis = “mechanism for the uptake of fluid and solutes into a cell”. It can be found on p. 48 in module 3, 3c2 Biology 130 Introductory Cell Biology Notes 2007 version. 2. Yes, Endocytosis is in the glossary. The definition is: “mechanism for the uptake of fluid and solutes into a cell. Can be divided into two types: bulk-phase endocytosis, which is non-specific, and receptor-mediated endocytosis, which requires the binding of solute molecules such as LDL or transferring to a specific cell-surface receptor.” It is mentioned on p. 311 of the course text book, Cell and Molecular Biology 5th edition. 3. Oleg Shupliakov, et al. (2007). Synaptic Vesicle Endocytosis Impaired by Disruption of Dynamin-SH3 Domain Interactions. [Electronic Version]. American Association for the Advancement of Science. Retrieved November 4, 2007, 2007, from: http://www.sciencemag.org/cgi/reprint/276/5310/259.pdf 4a. For the above article, the important background information could be summarized as follows. 1) The guanosine triphosphatase dynamin play an essential role in endocytosis as it

forms a collar at the neck of endocytic pits, and it is involved in fission reaction where it produces a free vesicle. 2) Through extinct binding sites, dynamin interacts with various Sh3 domaincontaining proteins. 4b. The purpose is to provide the evidence of the importance of interactions of dynamin with various SH3 domain-containing proteins for dynamin function in cells. 4c. Dynamin peptide was microinjected to exclude the chances of GSTamphSH3 impaired dynamin function rather than competed with an endogenous SH3 binding site in living synapses. 4d. One of the major conclusions is that interaction of dynamin’s praline-rich domain with Sh3 domain play a essential role in the final steps of the clathrin-coated vesicle formation. 5. Endocytosis comes from section 8.8, Cell and Molecular Biology. The fact that the article mentioned coated pits as the substances that enter a cell by RME will bound to receptors that collect in specialized domains of the plasma membrane can also be found in section 8.8. The terms clathrin adaptor AP-2, plasma membrane, and LDL can also be found in section 8.8.