Instrumentation Methods Of Analysis Rr

Set No. 1

Code No: RR222304

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 INSTRUMENTATION METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the instrumental methods? How are they classified? Name two or three instrumental methods for each of the physical property. [16] 2. (a) Explain and give examples of the types of transitions which occur inorganic compounds. (b) Arrange the following transitions in order of decreasing energy n → π∗, π → π∗, σ → σ∗ (c) What are the requirements for a solvent to be used in UV spectroscopy and why is ethanol considered as good solvent in UV. [8+3+5] 3. (a) What are the requirements for a spectrophotometric titration? What are the advantages of using a spectrophotometric titration? (b) How is the end point determined for a spectrophotometric titration.

[10+6]

4. (a) What are the advantages in atomic absorption of a heated graphite atomiser over a flame atomiser (b) Why are spectral interferences less severe in atomic absorption and atomic fluorescence spectroscopy than in flame emission spectroscopy? (c) What is the purpose of densitometer and how is it used in quantitative determination [4+6+6] 5. (a) Explain how the out put is detected. (b) Explain how two lines are separated. 6. What is nmr? Explain the properties of nuclei 7. What are the principal analytical uses of column chromatography?

[8+8] [16] [16]

8. How solvent extraction is useful for chemical separations. Discuss with suitable examples. [16] ⋆⋆⋆⋆⋆

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Set No. 2

Code No: RR222304

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 INSTRUMENTATION METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. What are the instrumental methods? How are they classified? Name two or three instrumental methods for each of the physical property. [16] 2. (a) Explain and give examples of the types of transitions which occur inorganic compounds. (b) Arrange the following transitions in order of decreasing energy n → π∗, π → π∗, σ → σ∗ (c) What are the requirements for a solvent to be used in UV spectroscopy and why is ethanol considered as good solvent in UV. [8+3+5] 3. (a) Why are complexes used to quantitatively determine metal ions? (b) What are the requirements of complex ions to be used in a spectrophotometric determinations? [8+8] 4. (a) Fluctuation in flame temperature can be considered a physical interference. Discuss the relative seriousness of flame temperature fluctuations in flame emission and atomic absorption spectroscopy. (b) Why so turbulent flow burners never strike back? (c) How will you determine lead in petrol by of atomic absorption spectroscopy [6+4+6] 5. (a) Explain the function of a monochromater. (b) How plasma emission is used for analytical purpose?

[8+8]

6. Write notes on: (a) Spin angular momentum (b) Larmor equation (c) Saturation (d) Homogeneous field

[16]

7. Discuss applications of ESR with suitable examples.

[16]

8. Describe three detectors with diagrams used in GC.

[16]

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Set No. 3

Code No: RR222304

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 INSTRUMENTATION METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. (a) How is relative error expressed? (b) Define the term mean and median. Under what condition does the median become equal to the mean? (c) What is the difference between atomic and molecular spectroscopy? (d) What is the effect of determinate error on normal error curve?

[4+4+4+4]

2. (a) Describe a double beam UV spectrophotometer with a block diagram. (b) What are the advantages of double beam UV spectrophotometer?

[10+6]

3. (a) What are the requirements for a spectrophotometric titration? What are the advantages of using a spectrophotometric titration? (b) How is the end point determined for a spectrophotometric titration.

[10+6]

4. Write short notes on: (a) Total consumption burner (b) Premix burner (c) Nebulizers.

[6+6+4]

5. (a) Explain preburn time with examples. (b) Matrix effects in emission spectroscopy.

[8+8]

6. What is chemical shift? How it is measured? Explain its significance with suitable examples [16] 7. Discuss ESR instrumentation with a block diagram.

[16]

8. Discuss theory and practice of HPLC.

[16]

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Set No. 4

Code No: RR222304

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007 INSTRUMENTATION METHODS OF ANALYSIS (Bio-Technology) Time: 3 hours Max Marks: 80 Answer any FIVE Questions All Questions carry equal marks ⋆⋆⋆⋆⋆ 1. (a) Distinguish between accuracy and precision. (b) What are determinate and indeterminate errors? (c) Write briefly about determinate and indeterminate errors. (d) Distinguish between relative and absolute error.

[3+3+6+4]

2. (a) What is photoelectric effect ? Discuss its theory? (b) What are photoelectric cells ? Describe their construction and working?[8+8] 3. (a) Why are complexes used to quantitatively determine metal ions? (b) What are the requirements of complex ions to be used in a spectrophotometric determinations? [8+8] 4. Write short notes on: (a) Total consumption burner (b) Premix burner (c) Nebulizers.

[6+6+4]

5. Write notes on (a) Direct reading spectrometers (b) Flame excitation (c) Interferences (d) Temperature of the plasma.

[16]

6. Write short notes on (a) Chemical shift (b) Frequency lock (c) Double resonance (d) Applications of proton nmr

[16]

7. Discuss applications of ESR with suitable examples. 8. Write notes on: (a) Gradient Elution 1 of 2

[16]

Set No. 4

Code No: RR222304 (b) Thermal Conductivity Detector (c) Reverse phase chromatography (d) Electron Capture Detector.

[16] ⋆⋆⋆⋆⋆

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