Fraction2 Volumes Mab (all)
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FR2 Samples Left to Run for the GPC MAB0905 Station 1-000 1-200 1-integrated 2-000 2-1000 3-000 3-200 4-000 4-065 5-000 5-043-pre 6-000-pre 7-000-pre 8-000-pre 9-000-pre 10-000-pre 10-026-pre 11-000-pre 11-026-pre 12-000-pre 12-019-pre 7p-000-pre blk MAB0406 1-000 1-200 4-000-pre 4-065-pre 5-000-pre 5-043-pre 10-000-pre 10-026-pre 11-000-pre 11-020-pre 12-000-pre 12-018-pre b1-28-000-pre b1-14-000-pre b2-2-000-1 b2-2-000-2 b3-0-000-1 b3-0-000-2 MAB0806 1-000-pre 1-017-pre 2-000-pre
Approximate Volume Left 1.8 ** 2.3 4 **
2.3 2.3 4
0.5 1.1 3.2 2 1 0.2 0.4 2.5 3 3 3.75 3.8 3.75 3.75 4.2 4 4 3.5 3.4 2.6 4.1 3.6 4.5 4.5 4.5 4.5 4.5 4.8
2-026-pre 4-000-pre 4-075-pre 7-000-1-pre 7-000-2-pre 7-1000-1-pre 7-1000-2-pre 8-000-pre 8-200-pre 10-000-pre 10-035-pre 11-000-pre 11-025-pre 12-000-pre 14-000-pre 16-000-1-pre 16-000-2-pre 19-000-1-pre 19-000-2-pre 20-000-pre 21-000-pre MAB1006 101406mb-B - surf 101406-ub-A - surf 101206B - B28 - surf 1-1000 2-000 2-deep 3-000 3-030 4-000 4-025 5-000 5-111 6-000 6-021 7-000 7-020 MAB1206 2-0 2-50 3-30 (R1) 3-30 (R2) 4-20 (R1) 4-20 (R2) 6-0 6-15 7-0 (R1) 7-0 (R2) 8-0 (R1)
8.2 9 4.6 3 3 4.3 5.8 4 5.8 6 6 4 6 4 6.2 4.2 2?
8-0 (R2) 9-0 9-20 11-0 (R1) 11-0 (R2) 13-0 14-0 (R1) 14-0 (R2)
9169764.xls 11/23/2207 Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 all stations recalculated by rdv using peak area from GC files and '200/300*5ml/20L' new data in peak area units (ng/uL) FR2 microw GC (Digestion) ave vol water FR2 (ul) total lignin Station comments vol (L) vol (ml) N used (Λ) (ug/L) S/V 1-000-pre 20 5.6 9/6/2007 2 600 0.74 1-200-pre 15 5.6 9/6/2007 2 800 0.71 1-40m integrated? 20 8.7 9/6/2007 2 600 1.68 2-000-pre 20 3 9/6/2007 2 800 0.36 2-1000-pre 20 3 8/29/2007 2 600 0.79 3-000-pre 20 5.2 8/22/2007 2 600 0.84 3-200-pre 20 8.3 8/22/2007 2 600 0.91 4-000-pre 20 8.4 10/9/2007 2 600 1.27 4-065-pre 20 5 8/17/2007 2 600 1.29 5-000-pre 20 3 10/3/2007 2 500 0.7 5-043-pre 20 5 10/3/2007 2 500 0.82 6-000-pre 20 3.5 1/19/07 (1/17) 3 200 3.29 7-000-pre (not fractionated) 20 8.3 1/19/07 (1/18) 3 200 10.24 8-000-pre 20 4 1/10/07 (1/8-10) 3 200 5.31 9-000-pre 20 6 12/14/06 (12/13) 2 196 5.55 10-000-pre 20 5.4 8/14/2007 2 600 1.16 10-026-pre 20 4.9 8/14/2007 2 600 1.62 11-000-pre 20 5.5 1/25/07 (1/22) 3 400 1.8 11-026-pre 20 5.5 2/8/07 (2/5) 2 600 1.78 12-000-pre 20 5 1/25/07 (1/23) 3 400 2.38 12-019-pre 20 5.5 2/8/07 (2/7) 2 600 2.24 7p-000-pre 20 8 12/14/06 (12/11-12)2 200 9.9 blank QH20 5-000-pre 6-000-pre 7-000-pre 7-000-pre 8-000-pre 8-000-pre 7p-000-pre
2.3 3.66 2.08 1.61 1.8 1.13 1.31
total lignin C/V [Ad/Al]v [Ad/Al]s [Ad/Al]v [Ad/Al]s (Λ) stdev S/V stdev stdev stdev stdev 1.01 0.81 5.27 0.03 0.17 0.03 0.18 0.94 1.15 1.21 0.7 0.06 0.08 0.1 0.19 0.03 0.84 2.05 7.24 0.79 0.13 0.35 1.9 6.72 1.63 0.6 4.41 0.04 0.89 0.42 0.4 1.13 1.27 0.62 19.28 0.05 0.36 0.16 0.16 1.51 1.23 0.48 3.65 0.14 0.12 0.1 0.3 0.31 1.62 1.02 3.16 0.08 0.12 0.01 0.05 0.47
2.13
1.11
1.43
8.06
0.06
0.3
0.04
0.96
0.96
2.62
1.97
0.75
4.05
0.27
0.28
0.74
0.12
0.69
2.1
1.16
1.56
3.8
0.16
0.09
0.26
0.62
1.37
1.25
1.23
4.07
0.03
0.16
0.09
0.21
0.22
1.07 1.1 1.12 0.98 2.02 2.15 0.93 0.78 1.01 0.99 1.17
0.28 0.28 0.31 0.29 1.03 0.83 0.22 0.22 0.33 0.3 0.32
0.83 0.89 1.03 1.06 0.83 1.86 0.95 1.01 0.95 0.97 1.06
1.4 1.2 1.2 1.16 2.28 5.36 1.37 1.74 1.46 1.71 1.35
0.05 0.86 0.21 0.17 0.07 0.65 0.16 0.38 0.04 0.08 1.89
0.04 0.03 0.02 0.02 0.09 0.03 0.04 0.01 0.02 0.04 0
0.01 0.02 0 0.01 0.27 0.27 0.01 0 0.07 0.01 0.02
0.01 0.04 0.14 0.05 0.01 1.51 0.01 0.1 0 0.11 0.08
0.07 0.08 0.1 0.01 0.08 4.67 0.12 0.1 0.02 0.22 0.24
2.03 1.07 0.89 1.13 1.27 1.16
1 0.26 0.55 0.29 0.49 0.66
0.69 0.86 0.6 1.14 0.83 0.32
1.53 1.07 0.73 1.44 1.23 0.77
0.12 0.18 0.85 0.34 0.27 0.11
0.14 0.1 0.04 0.05 0.05 0.15
0.03 0.02 0.07 0.01 0.01 0.09
0.14 0.04 0.02 0.35 0.08 0.42
0.75 0 0.06 0.28 0.16 0.01
0.3
4.7 20 20 20 20 20 20 20
3 10/11/2007 3.5 8/14/2007 8.3 connected 8.3 10/8/2007 4 1/10/07 (1/9) 4 10/9/2007 8 10/8/2007
1 2 2 2 2 2 2
300 200 200 400 200 300 400
2.42 9.31 3.73 5.04 2.4 3.23
Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 values recalculated by rdv using ng/uL provided by lynne and 200/300*5ml/20L new data in ug/L units (ng/uL) FR2 microw GC (Digestion) ave vol water FR2 (ul) total lignin Station comments vol (L) vol (ml) N used (Λ) (ug/L) S/V 1-000-pre 20 5.6 9/6/2007 2 600 0.69 1-40m 20 8.7 9/6/2007 2 600 1.83 1-200-pre 15 5.6 9/6/2007 2 800 0.85 2-000-pre 20 3 9/6/2007 2 800 0.34 2-1000-pre 20 3 8/29/2007 2 600 0.66 3-000-pre 20 5.2 8/22/2007 2 600 1.22 3-200-pre 20 8.3 8/22/2007 2 600 0.95 4-000-pre 20 8.4 10/9/2007 2 600 1.12 4-065-pre 20 5 8/17/2007 2 600 0.74 5-000-pre 20 3 10/3/2007 2 500 0.59 5-043-pre 20 5 10/3/2007 2 500 1.43 6-000-pre 20 3.5 1/19/07 (1/17) 3 200 4.12 7-000-pre (not fractionated) 20 8.3 1/19/07 (1/18) 3 200 11.62 8-000-pre 20 4 1/10/07 (1/8-10) 3 200 6.57 9-000-pre 20 6 12/14/06 (12/13) 2 196 7 10-000-pre 20 5.4 8/14/2007 2 600 1.03 10-026-pre 20 4.9 8/14/2007 2 600 1 11-000-pre 20 5.5 1/25/07 (1/22) 3 400 2.05 11-026-pre 20 5.5 2/8/07 (2/5) 2 600 1.78 12-000-pre 20 5 1/25/07 (1/23) 3 400 3.02 12-019-pre 20 5.5 2/8/07 (2/7) 2 600 2.51 7p-000-pre 20 8 12/14/06 (12/11-12)2 200 11.81 blank QH20 5-000-pre 6-000-pre 7-000-pre 7-000-pre 8-000-pre 8-000-pre 7p-000-pre
C/V
0.16 0.35 0.2 0.08 0.09 0.19 0.31 0.27 0.08 0.07 0.13 0.19 0.46 0.21 0.26 0.2 0.1 0.12 0.07 0.12 0.09 0.42
total lignin C/V [Ad/Al]v [Ad/Al]s [Ad/Al]v [Ad/Al]s (Λ) stdev S/V stdev stdev stdev stdev 0.1 0.06 0.02 0.01 0.03 0 0 0.01 0.18 0.11 0.03 0.82 0.16 0.03 0.01 0.04 0.12 0.1 0.02 0 0.02 0.01 0.02 0.01 0.05 0.04 0.01 0 0.01 0 0.01 0.01 0.06 0.03 0.88 0.04 0.02 0.01 0.01 0.17 0.09 0.05 0.04 0.39 0.02 0 0 0.01 0.19 0.13 0.11 0.06 0.02 0.01 0.02 0 0.28 0 0.24 0.08 0.04 0.01 0 0.34 0.16 0.05 0.18 0.22 0 0.1 0.02 0.12 0.06 0.04 0.07 0.13 0.01 0.01 0.01 0 0.08 0.07 0.17 0.11 0.02 0 0.01 0.01 0.04 0.14 0.23 0.01 0.01 0 0 0.01 0.1 0.38 0.47 0.29 0.01 0.01 0.02 0.03 0.05 0.18 0.25 0.03 0 0 0.03 0.02 0.05 0.28 0.35 0.11 0.01 0 0.02 0 0.1 0.05 0.03 0.02 0 0 0 0 0.06 0.05 0.2 0.22 0.05 0.01 0 0.06 0.01 0.11 0.21 0.06 0.01 0 0 0.02 0.01 0.08 0.17 0.14 0 0 0.01 0.01 0.03 0.1 0.2 0.17 0 0.01 0 0 0.02 0.08 0.16 0.04 0 0 0.01 0.02 0.08 0.37 0.53 1.44 0 0 0.03 0.11
0.14 0.11 0.46 0.15 0.21 0.22 0.17
0.12 0.11 0.08 0.12 0.04 0.1 0.11
C/V
4.7 20 20 20 20 20 20 20
3 10/11/2007 3.5 8/14/2007 8.3 connected 8.3 10/8/2007 4 1/10/07 (1/9) 4 10/9/2007 8 10/8/2007
1 2 2 2 2 2 2
300 200 200 400 200 300 400
0.89 2.77 11.83 2.67 6.27 1.47 2.19
0.05 0.16 0.32 0.19 0.21 0.02 0.18
0.32 0.2 0.4 0.37 0.29 0.41 0.39
0.44 0.39 0.31 0.55 0.29 0.28 0.52
0.01 0 0.04 0 0.01 0.01 0
0.03 0.01 0.01 0.01 0 0.02 0
0.01 0 0.01 0 0.07 0.01 0.01
0.1 0.01 0 0.02 0.06 0.04 0.01
Prepared by rdv 10/17/2008
Page 4
9169764.xls Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 new data together
Each run digested individually 200/300*(5ml/20L)
units (ng/uL) FR2 microw GC (Digestion) ave vol water FR2 (ul) Station comments vol (L) vol (ml) N used VAL VON VAD SAL 1-000-pre 20 5.6 9/6/2007 2 600 1.05 0.43 0.66 20 5.6 600 0.86 0.39 0.57 1-40m 20 8.7 9/6/2007 2 600 1.09 0.42 0.81 20 8.7 600 1.18 0.87 0.94 1-200-pre 15 5.6 9/6/2007 2 800 0.71 0.55 0.85 15 5.6 800 0.77 0.51 0.74 2-000-pre 20 3 9/6/2007 2 800 0.71 0.55 0.85 20 3 800 0.77 0.51 0.74 2-1000-pre 20 3 8/29/2007 2 600 0.53 2.11 0.22 20 3 600 0.36 3.24 0.27 3-000-pre 20 5.2 8/22/2007 2 600 1 0.85 0.61 20 5.2 600 1.17 2.37 0.67 3-200-pre 20 8.3 8/22/2007 2 600 0.47 0.62 0.52 20 8.3 600 0.48 0.5 0.41 4-000-pre 20 8.4 10/9/2007 2 600 0.94 0.73 0 20 8.4 600 0.93 0.65 0 4-065-pre 20 5 8/17/2007 2 600 1.34 1.57 0.63 20 5 600 0.55 0.46 0.47 5-000-pre 20 3 10/3/2007 2 500 1.3 1.04 1.07 20 3 500 1.17 0.79 0.77 20 3 10/11/2007 1 300 1.19 0.74 0.58 5-043-pre 20 5 10/3/2007 2 500 2.14 1.65 1.38 20 5 500 1.56 1.54 1.16 6-000-pre 20 3.5 1/19/07 (1/17) 3 200 3.99 3.14 3.16 20 3.5 200 4.02 3.28 3.12 20 3.5 200 3.99 3.19 3.08 20 3.5 8/14/2007 2 200 2.51 1.38 2.28 20 3.5 200 3.22 1.82 2.89 7-000-pre (not fractionated) 20 8.3 1/19/07 (1/18) 3 200 4.78 3.43 4.22 20 8.3 200 4.65 3.55 3.83 20 8.3 200 4.07 3.05 4.1 20 8.3 connected 2 200 4.91 3.76 3.95 20 8.3 200 4.99 3.41 3.76 20 8.3 10/8/2007 2 400 2.34 1.9 2.19 20 8.3 400 1.68 1.42 1.57 8-000-pre 20 4 1/10/07 (1/8-10) 3 200 5.14 3.82 5.27 20 4 200 5.38 4.18 4.53 20 4 200 5.62 4.44 4.79 20 4 1/10/07 (1/9) 2 200 4.66 4.07 5.9 20 4 200 5.32 3.69 4.24 20 4 10/9/2007 2 300 1.89 0.88 0.21 20 4 300 2.07 1.09 0.4 9-000-pre 20 6 12/14/06 (12/13) 2 196 4.12 3.29 3.94 20 6 196 4.18 3.19 3.71 10-000-pre 20 5.4 8/14/2007 2 600 1.53 0.26 0.82 vol was 500; changes to 600 20 5.4 600 1.65 0.28 0.83 10-026-pre 20 4.9 8/14/2007 2 600 1.86 1.3 1.04 20 4.9 600 1.03 2.43 0.58 11-000-pre 20 5.5 1/25/07 (1/22) 3 400 2.71 2.43 2.28 20 5.5 400 2.69 2.5 2.23 20 5.5 400 3.38 2.51 2.33 11-026-pre 20 5.5 2/8/07 (2/5) 2 600 3.54 3.72 3.51 20 5.5 600 3.46 3.43 2.94 12-000-pre 20 5 1/25/07 (1/23) 3 400 3.79 3.18 3.17 20 5 400 4.35 3.4 3.64 20 5 400 4.6 3.37 3.5 12-019-pre 20 5.5 2/8/07 (2/7) 2 600 4.33 4.17 4.13 20 5.5 600 4.7 3.69 3.81 7p-000-pre 20 8 12/14/06 (12/11-12)2 200 5.5 3.91 4.74 20 8 200 4.35 3.28 4.22 20 8 10/8/2007 2 400 1.54 0.81 1.43 20 8 400 2.26 1.21 1.89 blank QH20 4.7
Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 old data GC (Digestion) seawater FR2 Station vol (L) vol (ml) 1-000-pre 20 5.6 1-200-pre 15 5.6
SON 2.34 2.21 2.86 5.72 3.04 2.87 3.04 2.87 0.27 0.34 3.33 4.74 1.58 1.5 0.47 0 0.59 0.47 1.47 1 0.48 1.58 1.48 3.52 3.52 3.75 1.23 1.44 4.88 4.37 4.17 4.85 5.42 0.93 0.68 5.02 4.9 4.89 4.17 4.7 0.64 0.77 3.41 3.61 3.32 3.42 0.68 1 1.91 2.04 2.02 2.26 2.07 2.9 3.35 3.36 3.07 3.58 5.41 3.88 0.58 0.79
SAD 0.67 0.66 0.86 1.75 0.87 0.86 0.87 0.86 0.25 0.25 1.3 1.29 0.56 0.51 1.47 1.26 1.11 0.45 1.31 0.95 1.45 1.66 2.14 2.69 2.67 2.63 1.5 1.85 3.87 3.49 3.67 3.45 3.71 2.24 1.53 3.95 3.81 4.21 3.77 3.79 2.17 2.74 2.32 1.88 1.23 1.33 1.44 3.03 1.52 1.37 1.39 1.54 1.34 2.27 2.51 2.29 2.89 2.83 3.44 2.8 1.44 2.16
CAD 0.22 0.69 0.05 2.09 0.29 0.89 0.29 0.89 5.32 5.22 1.2 2.61 1.26 1.18 1.63 1.52 1.88 0.58 1.76 1.23 1.5 2.8 2.43 4.95 4.59 4.52 1.33 1.72 5.23 5.13 4.73 4.74 5.27 1.61 1.28 5.57 6.1 6.84 6.84 5.76 1.83 2.53 3.93 4.2 1.15 1.22 1.35 2.98 2.7 3.23 3.31 4.34 3.79 4.41 5.17 5.61 5.98 5.74 6.16 5.91 1.16 1.51
total lignin (Λ)
FAD 1.14 1 1.28 2.38 1.48 1.3 1.48 1.3 0.65 0.61 1.34 1.37 0.64 0.63 1.06 1.08 1.15 0.82 1.9 1.24 0.96 1.92 2.03 1.1 1.13 1.17 1.66 1.89 1.43 1.52 1.5 1.29 1.33 1.29 0.91 1.64 1.74 1.98 1.71 1.52 0.89 1.11 1.35 1.13 0.89 0.88 0.78 1.44 0.66 0.8 0.71 1.22 0.87 1.1 1.25 1.11 1.25 1.17 1.63 1.54 0.73 1.01
1 0.9 1.24 1.67 1.3 1.17 1.3 1.17 3.34 3.56 1.31 3.03 1.51 1.33 2.13 2.13 2.53 3.2 1.6 1.18 2 1.94 1.18 1.02 1.15 1.17 2.39 2.57 1.22 1.51 1.56 1.03 1.15 2.27 1.94 1.5 1.44 1.85 1.24 1.26 0.98 1.8 0.75 0.71 2.08 2.06 1.95 1.66 0 0 0 0.41 0.46 1.84 0.97 1.24 1.89 1.57 1.26 0.99 1.43 1.98
S/V
0.7 0.68 1.25 2.41 0.85 0.85 0.34 0.34 0.63 0.69 0.95 1.5 0.99 0.9 1.18 1.06 0.9 0.58 0.69 0.5 0.89 1.51 1.35 4.13 4.11 4.11 2.5 3.05 12.06 11.64 11.14 11.61 12.05 3.06 2.29 6.38 6.42 6.92 6.47 6.06 1.27 1.67 7.07 6.92 1.02 1.05 0.85 1.16 1.95 2.04 2.15 1.88 1.68 2.83 3.08 3.14 2.54 2.48 12.82 10.79 1.82 2.56
C/V 0.14 0.18 0.24 0.46 0.18 0.21 0.07 0.09 0.1 0.08 0.21 0.18 0.29 0.32 0.3 0.25 0.08 0.08 0.08 0.07 0.14 0.12 0.14 0.19 0.18 0.19 0.12 0.11 0.47 0.45 0.46 0.43 0.49 0.15 0.16 0.2 0.21 0.21 0.2 0.22 0.21 0.23 0.26 0.27 0.2 0.19 0.07 0.14 0.11 0.12 0.11 0.07 0.07 0.12 0.12 0.12 0.09 0.09 0.42 0.42 0.17 0.17
total lignin [Ad/Al]v [Ad/Al]s (Λ) S/V 0.09 0.06 0.01 0.69 0.1 0.06 0.03 0.16 0.11 0 1.83 0.2 0.12 0.05 0.12 0.11 0.01 0.85 0.11 0.09 0.03 0.05 0.04 0 0.34 0.05 0.04 0.01 0.07 0.02 1 0.66 0.05 0.04 0.76 0.09 0.05 0.03 1.22 0.09 0.05 0.05 0.18 0.15 0.11 0.95 0.2 0.12 0.11 0.27 0 0.48 1.12 0.28 0 0 0.09 0.04 0.27 0.74 0.23 0.07 0.1 0.06 0.05 0.07 0.59 0.05 0.04 0.07 0.12 0.05 0.32 0.89 0.07 0.06 0.18 1.43 0.08 0.07 0.16 0.04 0.14 0.25 4.12 0.04 0.14 0.23 0.04 0.14 0.21 0.12 0.16 0.19 2.77 0.1 0.16 0.21 0.09 0.37 0.44 11.62 0.1 0.34 0.49 0.11 0.42 0.47 0.08 0.33 0.41 11.83 0.08 0.31 0.4 0.11 0.19 0.36 2.67 0.13 0.19 0.39 0.04 0.2 0.22 6.57 0.05 0.17 0.25 0.05 0.17 0.28 0.04 0.25 0.33 6.27 0.04 0.16 0.25 0.08 0.01 0.38 1.47 0.11 0.03 0.44 0.06 0.29 0.35 7 0.05 0.27 0.36 0.1 0.05 0.03 1.03 0.1 0.05 0.03 0.05 0.05 0.16 1 0.06 0.05 0.24 0.01 0.12 0.19 2.05 0.01 0.11 0.22 0.01 0.09 0.23 0.01 0.09 0.18 1.78 0.01 0.08 0.17 0.04 0.1 0.19 3.02 0.02 0.1 0.19 0.03 0.1 0.21 0.02 0.09 0.18 2.51 0.02 0.07 0.15 0.08 0.34 0.46 11.81 0.09 0.39 0.61 0.11 0.19 0.4 2.19 0.11 0.17 0.38
C/V 0.16
total lignin [Ad/Al]v [Ad/Al]s [Ad/Al]v [Ad/Al]s (Λ) stdev S/V stdev C/V stdev stdev stdev 0.1 0.06 0.02 0.01 0.03 0 0 0.01
0.35
0.18
0.11
0.03
0.82
0.16
0.03
0.01
0.04
0.2
0.12
0.1
0.02
0
0.02
0.01
0.02
0.01
0.08
0.05
0.04
0.01
0
0.01
0
0.01
0.01
0.09
0.06
0.03
0.88
0.04
0.02
0.01
0.01
0.17
0.19
0.09
0.05
0.04
0.39
0.02
0
0
0.01
0.31
0.19
0.13
0.11
0.06
0.02
0.01
0.02
0
0.27
0.28
0
0.24
0.08
0.04
0.01
0
0.34
0.08
0.16
0.05
0.18
0.22
0
0.1
0.02
0.12
0.07
0.06
0.04
0.07
0.13
0.01
0.01
0.01
0
0.14 0.13
0.12 0.08
0.05 0.07
0.32 0.17
0.44 0.11
0.01 0.02
0.03 0
0.01 0.01
0.1 0.01
0.19
0.04
0.14
0.23
0.01
0.01
0
0
0.01
0.11
0.11
0.16
0.2
0.39
0
0.01
0
0.01
0.46
0.1
0.38
0.47
0.29
0.01
0.01
0.02
0.03
0.46
0.08
0.32
0.4
0.31
0.04
0.01
0.01
0
0.15
0.12
0.19
0.37
0.55
0
0.01
0
0.02
0.21
0.05
0.18
0.25
0.03
0
0
0.03
0.02
0.21
0.04
0.21
0.29
0.29
0.01
0
0.07
0.06
0.22
0.1
0.02
0.41
0.28
0.01
0.02
0.01
0.04
0.26
0.05
0.28
0.35
0.11
0.01
0
0.02
0
0.2
0.1
0.05
0.03
0.02
0
0
0
0
0.1
0.06
0.05
0.2
0.22
0.05
0.01
0
0.06
0.12
0.01
0.11
0.21
0.06
0.01
0
0
0.02
0.07
0.01
0.08
0.17
0.14
0
0
0.01
0.01
0.12
0.03
0.1
0.2
0.17
0
0.01
0
0
0.09
0.02
0.08
0.16
0.04
0
0
0.01
0.02
0.42
0.08
0.37
0.53
1.44
0
0
0.03
0.11
0.17
0.11
0.18
0.39
0.52
0
0
0.01
0.01
Analysis year 2006&2007 Each run digested individually units (µg/L) N
VAL
VAD/VAL SAD/SAL VON
VAD
V
SAL
SON
SAD
S
CAD
FAD
C
Prepared by rdv 10/17/2008
total lignin (Λ) S/V
C/V
[Ad/Al]v
[Ad/Al]s
Page 5
9169764.xls 1-integrated 2-000-pre 2-1000-pre 3-000-pre 3-200-pre 4-000-pre 4-065-pre 5-000-pre 5-043-pre 6-000-pre 7-000-pre 8-000-pre
20? ? ? 20 20 20 20 20 20 20 20 20
9-000-pre 10-000-pre 10-026-pre 11-000-pre 11-026-pre 12-000-pre 12-019-pre 7p-000-pre blank QH20
20 20 20 20 20 20 20 20
8.7 ? 3 5.2 8.3* 8.4 2/2/07(2/1) 5 ? 5 3.5* 1/19/07 (1/17) 3 8.3 1/19/07 (1/18) 3 4* 1/10/07 (1/8-10) 3 1/10/07 (1/9) 2 <6 12/14/06 (12/13) 2 5.4 4.9 5.5 1/25/07 (1/22) 3 5.5 2/8/07 (2/5) 2 5* 1/25/07 (1/23) 3 5.5 2/8/07 (2/7) 2 <8 12/14/06 (12/11-12)3 4.7
0.700± 0.003 .56± .01 .547± .0071.81± .01 .63± .02 1.87± .16 1.39± .11 1.68± .08 4.94± .26 1.86± .15 1.08±.05 .83±.06 .97±.08 2.88±.08 .99±.01 0.10± .1 .78± .05 1.01± .23 2.79± .19 .89± .07 1.27± 0.01 .99±.02 1.16±.05 3.43±.06 1.07±0.04
.47± .01 .82± .04 1.53± .08 2.09± .11 .80±.04 1.23±.13 .757± .003 1.26± .15 .6±.1 1.24±.06
1.92± .04 5.47± .3 3.02±.15 2.90± .07 2.96±.01
.199± .006 .20± .02 .93± .02 4.12± .01 .62± .02 .59± .08 1.21±.0911.6± .5 .36±.03 .32±.04 .68±.08 6.6±.3 0.32± .03 .251± .003 .57± .02 6.27±.29 .38±.05 .22±.01 .60±.06 7.0±.1
1.06± .03 1.11± .02 1.05±.03 1.04± .05 .86±.02
.22± .01 .78± .01 .25± .03 .90± .09 .23±.02 .91±.10 .206± .006 1.0± .3 .17±.01 .92±.05
1.3± .1 1.13± .05 1.25±.14 1.4± .3 1.16±.01
0.40± .05 0.321 ± .005 .53± .05 .41 ± .02 1.93±.24
.341± .006.314± .0071.06± .06 .27± .01 0.33 ± .020.30 ± .04.94 ± .06 .20 ± .01 .41± .02 .43± .03 1.38± .09 .40± .03 .36 ± .03 .36 ± .02 1.14 ± .03.30 ± .03 1.42±.13 1.85±.14 5.21±.46 1.76±.35
.20± .01 .42± .05 .13 ± .01 .37 ± .04 .29±.02 .63± .08 .262 ± .004.54 ± .02 1.22±.13 2.44±.06
.89± .04 .10± .01 ND .10 ± .012.05 ± .10 .70 ± .06 .10 ±.02 .040 ±.004 .14 ±.02 1.78 ± .14 1.33± .11 .14± .01 .16± .06 .31± .05 3.02± .16 1.10 ± .01.111 ± .005.16 ± .02 .27 ± .032.51 ±.04 5.43±.51 .65±.04 .42±.08 1.07±.0711.7±1.0
.85 ± .04 .74 ± .02 .96± .02 0.97 ± .04 1.04±.04
.09 ± .01 .79 ± .09 .14 ± .01 .92 ± .10 .23± .05 .81± .04 .24 ± .02 .88 ± .10 0.20±.0.01 .96±.09
1.55 ± .12 1.88 ± .07 1.58± .08 1.78 ± .25 1.41±.24
VON
SAD
FAD
needed to redo to adjust the final volume of py in GC vial!! st8 2 vessels connected to each other for digestion & used 300watts for the microwave
by Lynne
Station 1-000-pre 1-40m 1-200-pre 2-000-pre 2-1000-pre 3-000-pre 3-200-pre 4-000-pre 4-065-pre 5-000-pre
seawate microw r FR2 ave V vol (L) vol (ml) date of anaysis N (ul) VAL 20 5.6 9/6/2007 600 600 20? 8.7 9/6/2007 600 600 15 5.6 9/6/2007 800 800 ? 3 8/29/2007 500 500 ? 3 8/29/2007 600 600 20 5.2 8/22/2007 600 600 20 8.3* 8/22/2007 600 600 20 8.4 10/9/2007 600 600 20 5 8/17/2007 600 600 3 500 20 10/3/2007
3
5-043-pre
20 20
5
10/11/2007 10/3/2007
6-000-pre
20
3.5*
8/14/2007
7-000-pre
20
8.3
10/8/2007
8-000-pre
20
4*
10/9/2007
9-000-pre 10-000-pre
20 20
<6 12/14/06 (12/13) 5.4 8/14/2007
10-026-pre
20
4.9
8/14/2007
11-000-pre
20
5.5
8/7/2007
11-026-pre
20
5.5
8/7/2007
12-000-pre 12-019-pre 7p-000-pre
20 20 20
5* 5.5 <8
10/8/2007
VAD
SAL
SON
CAD
1.05 0.86 1.09 1.18 0.71 0.77 0.53 0.76 0.53 0.36 1 1.17 0.47 0.48 0.94 0.93 1.34 0.55 1.3 1.17 1.19 2.14 1.56 2.51 3.22 2.34 1.68 1.89 2.07
0.43 0.39 0.42 0.87 0.55 0.51 0.18 0.2 2.11 3.24 0.85 2.37 0.62 0.5 0.73 0.65 1.57 0.46 1.04 0.79 0.74 1.65 1.54 1.38 1.82 1.9 1.42 0.88 1.09
0.66 0.57 0.81 0.94 0.85 0.74 0.29 0.36 0.22 0.27 0.61 0.67 0.52 0.41 0 0 0.63 0.47 1.07 0.77 0.58 1.38 1.16 2.28 2.89 2.19 1.57 0.21 0.4
2.34 2.21 2.86 5.72 3.04 2.87 0.41 0.34 0.27 0.34 3.33 4.74 1.58 1.5 0.47 0 0.59 0.47 1.47 1 0.48 1.58 1.48 1.23 1.44 0.93 0.68 0.64 0.77
0.67 0.66 0.86 1.75 0.87 0.86 0.46 0.18 0.25 0.25 1.3 1.29 0.56 0.51 1.47 1.26 1.11 0.45 1.31 0.95 1.45 1.66 2.14 1.5 1.85 2.24 1.53 2.17 2.74
0.22 0.69 0.05 2.09 0.29 0.89 1.1 2.39 5.32 5.22 1.2 2.61 1.26 1.18 1.63 1.52 1.88 0.58 1.76 1.23 1.5 2.8 2.43 1.33 1.72 1.61 1.28 1.83 2.53
1.14 1 1.28 2.38 1.48 1.3 0.73 0.63 0.65 0.61 1.34 1.37 0.64 0.63 1.06 1.08 1.15 0.82 1.9 1.24 0.96 1.92 2.03 1.66 1.89 1.29 0.91 0.89 1.11
1 0.9 1.24 1.67 1.3 1.17 1.69 1.55 3.34 3.56 1.31 3.03 1.51 1.33 2.13 2.13 2.53 3.2 1.6 1.18 2 1.94 1.18 2.39 2.57 2.27 1.94 0.98 1.8
600 500 600 600 200 200 200 200
1.53 1.65 1.86 1.03
0.26 0.28 1.3 2.43
0.82 0.83 1.04 0.58
3.32 3.42 0.68 1
1.23 1.33 1.44 3.03
1.15 1.22 1.35 2.98
0.89 0.88 0.78 1.44
2.08 2.06 1.95 1.66
400 400
1.54 2.26
0.81 1.21
1.43 1.89
0.58 0.79
1.44 2.16
1.16 1.51
0.73 1.01
1.43 1.98
500 300 500 500 200 200 400 400 300 300
by tammi Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2
Each run digested individually
GC (Digestion) Station 1-000-pre 1-200-pre 1-integrated
seawater FR2 vol (L) vol (ml) 20 5.6 15 5.6 20? 8.7
units (ng/uL) FR2 vol (ml) N used VAL
VAD/VAL SAD/SAL
VON
VAD
V
SAL
SON
SAD
S
Prepared by rdv 10/17/2008
CAD
FAD
C
total lignin (Λ) S/V
C/V
[Ad/Al]v
[Ad/Al]s
Page 6
9169764.xls 2-000-pre 2-1000-pre 3-000-pre 3-200-pre 4-000-pre 4-065-pre 5-000-pre 5-043-pre 6-000-pre
? ? 20 20 20 20 20 20 20
? 3 5.2 8.3* 8.4 2/2/07(2/1) 5 ? 5 3.5* 1/19/07 (1/17)
3
7-000-pre
20
8.3 1/19/07 (1/18)
3
8-000-pre
20
4* 1/10/07 (1/8-10)
3
1/10/07 (1/9)
2
9-000-pre
20
<6 12/14/06 (12/13)
2
10-000-pre 10-026-pre 11-000-pre
20 20 20
5.4 4.9 5.5 1/25/07 (1/22)
3
11-026-pre
20
5.5 2/8/07 (2/5)
2
12-000-pre
20
12-019-pre
20
5.5 2/8/07 (2/7)
7p-000-pre
20
<8 12/14/06 (12/11-12)3
connected
blank QH20
5* 1/25/07 (1/23)
3
2
200 200 200 200 200 200 200 200 200 200 200 200 200 196 196
3.99 4.02 3.99 4.78 4.65 4.07 4.91 4.99 5.14 5.38 5.62 4.66 5.32 4.12 4.18
3.14 3.28 3.19 3.43 3.55 3.05 3.76 3.41 3.82 4.18 4.44 4.07 3.69 3.29 3.19
3.16 3.12 3.08 4.22 3.83 4.1 3.95 3.76 5.27 4.53 4.79 5.9 4.24 3.94 3.71
3.52 3.52 3.75 4.88 4.37 4.17 4.85 5.42 5.02 4.9 4.89 4.17 4.7 3.41 3.61
2.69 2.67 2.63 3.87 3.49 3.67 3.45 3.71 3.95 3.81 4.21 3.77 3.79 2.32 1.88
4.95 4.59 4.52 5.23 5.13 4.73 4.74 5.27 5.57 6.1 6.84 6.84 5.76 3.93 4.2
1.1 1.13 1.17 1.43 1.52 1.5 1.29 1.33 1.64 1.74 1.98 1.71 1.52 1.35 1.13
1.02 1.15 1.17 1.22 1.51 1.56 1.03 1.15 1.5 1.44 1.85 1.24 1.26 0.75 0.71
400 400 400 600 600 400 400 400 600 600 200 200
2.71 2.69 3.38 3.54 3.46 3.79 4.35 4.6 4.33 4.7 5.5 4.35
2.43 2.5 2.51 3.72 3.43 3.18 3.4 3.37 4.17 3.69 3.91 3.28
2.28 2.23 2.33 3.51 2.94 3.17 3.64 3.5 4.13 3.81 4.74 4.22
1.91 2.04 2.02 2.26 2.07 2.9 3.35 3.36 3.07 3.58 5.41 3.88
1.52 1.37 1.39 1.54 1.34 2.27 2.51 2.29 2.89 2.83 3.44 2.8
2.7 3.23 3.31 4.34 3.79 4.41 5.17 5.61 5.98 5.74 6.16 5.91
0.66 0.8 0.71 1.22 0.87 1.1 1.25 1.11 1.25 1.17 1.63 1.54
0 0 0 0.41 0.46 1.84 0.97 1.24 1.89 1.57 1.26 0.99
4.7
needed to redo to adjust the final volume of py in GC vial!! st8 2 vessels connected to each other for digestion & used 300watts for the microwave
Prepared by rdv 10/17/2008
Page 7
Lignin-derived phenols MARS/ GC analysis MAB Cruise Apr. 2006 C18 SPE extractions FR2 units (µg/L) seawater FR2 Station vol (L) vol (ml) 1-000-pre 20 4.89 1-200-pre 20 4.78 4-000-pre 20 5.34 4-065-pre 20 4.97 5-000-pre 20 4.9 5-043-pre 20 5 10-000-pre 20 4.94 10-026-pre 20 4.95 11-000-pre 20 4.99 11-020-pre 20 4.99 12-000-pre 20 4.92 12-018-pre 20 4.99 b1-28-000-pre 20 4.91 b1-14-000-pre 20 4.95 b2-2-000-1 20 5* b2-2-000-2 20 5.07 b3-0-000-1 20 5.07 b3-0-000-2 20 4.96
GC Analysis Date
needed to redo to adjust the final volume!!
N
VAL
VON
VAD
V
SAL
SON
SAD
S
CAD
FAD
C
total lignin (Λ) S/V
C/V
VAD/VAL SAD/SAL [Ad/Al]v
[Ad/Al]s
Lignin-derived phenols MARS/ GC analysis MAB Cruise Apr. 2006 C18 SPE extractions FR2 units (µg/L) Station 1-000-pre 1-017-pre 2-000-pre 2-026-pre 4-000-pre 4-075-pre 7-000-1-pre 7-000-2-pre 7-1000-1-pre 7-1000-2-pre 8-000-pre 8-200-pre 10-000-pre 10-035-pre 11-000-pre 11-025-pre 12-000-pre 14-000-pre 16-000-1-pre 16-000-2-pre 19-000-1-pre 19-000-2-pre 20-000-pre 21-000-pre
seawater FR2 vol (L) vol (ml) 20 4.398 20 4.218 20 4.199 20 4.199 20 4.348 20 4.298 20 2.215 20 4.298 20 4.247 20 4.137 20 4.226 20 4.108 20 4.327 20 4.284 20 4.218 20 4.199 20 5.399 20 6.643 20 4.624 20 5.610 20 5.395 20 5.447 20 7.925 20 4.994 seater vol check!!
GC Analysis Date
N
VAL
VON
VAD
V
SAL
SON
SAD
S
CAD
FAD
C
total lignin (Λ) S/V
C/V
VAD/VAL SAD/SAL [Ad/Al]v
[Ad/Al]s
Station FR2 volume 101206-B28 5.1 101206-B28B 5.5 101306B26 9.2 101306B26A 9.54 101406MB-A 9.1 101406MB-B 9.25 101406UB-A 10.29 101406UB-B 9.54 101506-B0A 20.84 101506-B0B 10.98 1-1000 3.2 2-000 4.2 2-200 5.1 3-000 6.2 3-030 4.24 4-000 6.26 4-025 6.2 5-000 2.3 5-111 4.2 6-000 6.39 6-021 4.2 7-000 6.4 7-020 4.34
MAB1206 Station # Depth Fr2 Added 2 0 400 2 50 400 3 30 (R1) 500 3 30 (R2) 500 4 20 (R1) 500 4 20 (R2) 500 6 0 500 6 15 500 7 0 (R1) 300 7 0 (R2) 300 8 0 (R1) 300 8 0 (R2) 300 9 0 500 9 20 500 11 0 (R1) 400 11 0 (R2) 400 13 0 300 14 0 (R1) 400 14 0 (R2) 400
MAB Cruise Date Station # Depth FR2 Added MAB0806 19 0 200 MAB0806 8 0 400 MAB0806 11 surface 300 MAB0806 11 deep 300 MAB0905 11 surface 300 MAB0905 11 deep 300 MAB0905 6 0 100 MAB0905 7 original 100 MAB0905 8 0 50 MAB0905 9 0 100
Approximate Volume Left 1.8 ** 2.3 4 **
2.3 2.3 4
0.5 1.1 3.2 2 1 0.2 0.4 2.5 3 3 3.75 3.8 3.75 3.75 4.2 4 4 3.5 3.4 2.6 4.1 3.6 4.5 4.5 4.5 4.5 4.5 4.8
2-026-pre 4-000-pre 4-075-pre 7-000-1-pre 7-000-2-pre 7-1000-1-pre 7-1000-2-pre 8-000-pre 8-200-pre 10-000-pre 10-035-pre 11-000-pre 11-025-pre 12-000-pre 14-000-pre 16-000-1-pre 16-000-2-pre 19-000-1-pre 19-000-2-pre 20-000-pre 21-000-pre MAB1006 101406mb-B - surf 101406-ub-A - surf 101206B - B28 - surf 1-1000 2-000 2-deep 3-000 3-030 4-000 4-025 5-000 5-111 6-000 6-021 7-000 7-020 MAB1206 2-0 2-50 3-30 (R1) 3-30 (R2) 4-20 (R1) 4-20 (R2) 6-0 6-15 7-0 (R1) 7-0 (R2) 8-0 (R1)
8.2 9 4.6 3 3 4.3 5.8 4 5.8 6 6 4 6 4 6.2 4.2 2?
8-0 (R2) 9-0 9-20 11-0 (R1) 11-0 (R2) 13-0 14-0 (R1) 14-0 (R2)
9169764.xls 11/23/2207 Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 all stations recalculated by rdv using peak area from GC files and '200/300*5ml/20L' new data in peak area units (ng/uL) FR2 microw GC (Digestion) ave vol water FR2 (ul) total lignin Station comments vol (L) vol (ml) N used (Λ) (ug/L) S/V 1-000-pre 20 5.6 9/6/2007 2 600 0.74 1-200-pre 15 5.6 9/6/2007 2 800 0.71 1-40m integrated? 20 8.7 9/6/2007 2 600 1.68 2-000-pre 20 3 9/6/2007 2 800 0.36 2-1000-pre 20 3 8/29/2007 2 600 0.79 3-000-pre 20 5.2 8/22/2007 2 600 0.84 3-200-pre 20 8.3 8/22/2007 2 600 0.91 4-000-pre 20 8.4 10/9/2007 2 600 1.27 4-065-pre 20 5 8/17/2007 2 600 1.29 5-000-pre 20 3 10/3/2007 2 500 0.7 5-043-pre 20 5 10/3/2007 2 500 0.82 6-000-pre 20 3.5 1/19/07 (1/17) 3 200 3.29 7-000-pre (not fractionated) 20 8.3 1/19/07 (1/18) 3 200 10.24 8-000-pre 20 4 1/10/07 (1/8-10) 3 200 5.31 9-000-pre 20 6 12/14/06 (12/13) 2 196 5.55 10-000-pre 20 5.4 8/14/2007 2 600 1.16 10-026-pre 20 4.9 8/14/2007 2 600 1.62 11-000-pre 20 5.5 1/25/07 (1/22) 3 400 1.8 11-026-pre 20 5.5 2/8/07 (2/5) 2 600 1.78 12-000-pre 20 5 1/25/07 (1/23) 3 400 2.38 12-019-pre 20 5.5 2/8/07 (2/7) 2 600 2.24 7p-000-pre 20 8 12/14/06 (12/11-12)2 200 9.9 blank QH20 5-000-pre 6-000-pre 7-000-pre 7-000-pre 8-000-pre 8-000-pre 7p-000-pre
2.3 3.66 2.08 1.61 1.8 1.13 1.31
total lignin C/V [Ad/Al]v [Ad/Al]s [Ad/Al]v [Ad/Al]s (Λ) stdev S/V stdev stdev stdev stdev 1.01 0.81 5.27 0.03 0.17 0.03 0.18 0.94 1.15 1.21 0.7 0.06 0.08 0.1 0.19 0.03 0.84 2.05 7.24 0.79 0.13 0.35 1.9 6.72 1.63 0.6 4.41 0.04 0.89 0.42 0.4 1.13 1.27 0.62 19.28 0.05 0.36 0.16 0.16 1.51 1.23 0.48 3.65 0.14 0.12 0.1 0.3 0.31 1.62 1.02 3.16 0.08 0.12 0.01 0.05 0.47
2.13
1.11
1.43
8.06
0.06
0.3
0.04
0.96
0.96
2.62
1.97
0.75
4.05
0.27
0.28
0.74
0.12
0.69
2.1
1.16
1.56
3.8
0.16
0.09
0.26
0.62
1.37
1.25
1.23
4.07
0.03
0.16
0.09
0.21
0.22
1.07 1.1 1.12 0.98 2.02 2.15 0.93 0.78 1.01 0.99 1.17
0.28 0.28 0.31 0.29 1.03 0.83 0.22 0.22 0.33 0.3 0.32
0.83 0.89 1.03 1.06 0.83 1.86 0.95 1.01 0.95 0.97 1.06
1.4 1.2 1.2 1.16 2.28 5.36 1.37 1.74 1.46 1.71 1.35
0.05 0.86 0.21 0.17 0.07 0.65 0.16 0.38 0.04 0.08 1.89
0.04 0.03 0.02 0.02 0.09 0.03 0.04 0.01 0.02 0.04 0
0.01 0.02 0 0.01 0.27 0.27 0.01 0 0.07 0.01 0.02
0.01 0.04 0.14 0.05 0.01 1.51 0.01 0.1 0 0.11 0.08
0.07 0.08 0.1 0.01 0.08 4.67 0.12 0.1 0.02 0.22 0.24
2.03 1.07 0.89 1.13 1.27 1.16
1 0.26 0.55 0.29 0.49 0.66
0.69 0.86 0.6 1.14 0.83 0.32
1.53 1.07 0.73 1.44 1.23 0.77
0.12 0.18 0.85 0.34 0.27 0.11
0.14 0.1 0.04 0.05 0.05 0.15
0.03 0.02 0.07 0.01 0.01 0.09
0.14 0.04 0.02 0.35 0.08 0.42
0.75 0 0.06 0.28 0.16 0.01
0.3
4.7 20 20 20 20 20 20 20
3 10/11/2007 3.5 8/14/2007 8.3 connected 8.3 10/8/2007 4 1/10/07 (1/9) 4 10/9/2007 8 10/8/2007
1 2 2 2 2 2 2
300 200 200 400 200 300 400
2.42 9.31 3.73 5.04 2.4 3.23
Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 values recalculated by rdv using ng/uL provided by lynne and 200/300*5ml/20L new data in ug/L units (ng/uL) FR2 microw GC (Digestion) ave vol water FR2 (ul) total lignin Station comments vol (L) vol (ml) N used (Λ) (ug/L) S/V 1-000-pre 20 5.6 9/6/2007 2 600 0.69 1-40m 20 8.7 9/6/2007 2 600 1.83 1-200-pre 15 5.6 9/6/2007 2 800 0.85 2-000-pre 20 3 9/6/2007 2 800 0.34 2-1000-pre 20 3 8/29/2007 2 600 0.66 3-000-pre 20 5.2 8/22/2007 2 600 1.22 3-200-pre 20 8.3 8/22/2007 2 600 0.95 4-000-pre 20 8.4 10/9/2007 2 600 1.12 4-065-pre 20 5 8/17/2007 2 600 0.74 5-000-pre 20 3 10/3/2007 2 500 0.59 5-043-pre 20 5 10/3/2007 2 500 1.43 6-000-pre 20 3.5 1/19/07 (1/17) 3 200 4.12 7-000-pre (not fractionated) 20 8.3 1/19/07 (1/18) 3 200 11.62 8-000-pre 20 4 1/10/07 (1/8-10) 3 200 6.57 9-000-pre 20 6 12/14/06 (12/13) 2 196 7 10-000-pre 20 5.4 8/14/2007 2 600 1.03 10-026-pre 20 4.9 8/14/2007 2 600 1 11-000-pre 20 5.5 1/25/07 (1/22) 3 400 2.05 11-026-pre 20 5.5 2/8/07 (2/5) 2 600 1.78 12-000-pre 20 5 1/25/07 (1/23) 3 400 3.02 12-019-pre 20 5.5 2/8/07 (2/7) 2 600 2.51 7p-000-pre 20 8 12/14/06 (12/11-12)2 200 11.81 blank QH20 5-000-pre 6-000-pre 7-000-pre 7-000-pre 8-000-pre 8-000-pre 7p-000-pre
C/V
0.16 0.35 0.2 0.08 0.09 0.19 0.31 0.27 0.08 0.07 0.13 0.19 0.46 0.21 0.26 0.2 0.1 0.12 0.07 0.12 0.09 0.42
total lignin C/V [Ad/Al]v [Ad/Al]s [Ad/Al]v [Ad/Al]s (Λ) stdev S/V stdev stdev stdev stdev 0.1 0.06 0.02 0.01 0.03 0 0 0.01 0.18 0.11 0.03 0.82 0.16 0.03 0.01 0.04 0.12 0.1 0.02 0 0.02 0.01 0.02 0.01 0.05 0.04 0.01 0 0.01 0 0.01 0.01 0.06 0.03 0.88 0.04 0.02 0.01 0.01 0.17 0.09 0.05 0.04 0.39 0.02 0 0 0.01 0.19 0.13 0.11 0.06 0.02 0.01 0.02 0 0.28 0 0.24 0.08 0.04 0.01 0 0.34 0.16 0.05 0.18 0.22 0 0.1 0.02 0.12 0.06 0.04 0.07 0.13 0.01 0.01 0.01 0 0.08 0.07 0.17 0.11 0.02 0 0.01 0.01 0.04 0.14 0.23 0.01 0.01 0 0 0.01 0.1 0.38 0.47 0.29 0.01 0.01 0.02 0.03 0.05 0.18 0.25 0.03 0 0 0.03 0.02 0.05 0.28 0.35 0.11 0.01 0 0.02 0 0.1 0.05 0.03 0.02 0 0 0 0 0.06 0.05 0.2 0.22 0.05 0.01 0 0.06 0.01 0.11 0.21 0.06 0.01 0 0 0.02 0.01 0.08 0.17 0.14 0 0 0.01 0.01 0.03 0.1 0.2 0.17 0 0.01 0 0 0.02 0.08 0.16 0.04 0 0 0.01 0.02 0.08 0.37 0.53 1.44 0 0 0.03 0.11
0.14 0.11 0.46 0.15 0.21 0.22 0.17
0.12 0.11 0.08 0.12 0.04 0.1 0.11
C/V
4.7 20 20 20 20 20 20 20
3 10/11/2007 3.5 8/14/2007 8.3 connected 8.3 10/8/2007 4 1/10/07 (1/9) 4 10/9/2007 8 10/8/2007
1 2 2 2 2 2 2
300 200 200 400 200 300 400
0.89 2.77 11.83 2.67 6.27 1.47 2.19
0.05 0.16 0.32 0.19 0.21 0.02 0.18
0.32 0.2 0.4 0.37 0.29 0.41 0.39
0.44 0.39 0.31 0.55 0.29 0.28 0.52
0.01 0 0.04 0 0.01 0.01 0
0.03 0.01 0.01 0.01 0 0.02 0
0.01 0 0.01 0 0.07 0.01 0.01
0.1 0.01 0 0.02 0.06 0.04 0.01
Prepared by rdv 10/17/2008
Page 4
9169764.xls Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 new data together
Each run digested individually 200/300*(5ml/20L)
units (ng/uL) FR2 microw GC (Digestion) ave vol water FR2 (ul) Station comments vol (L) vol (ml) N used VAL VON VAD SAL 1-000-pre 20 5.6 9/6/2007 2 600 1.05 0.43 0.66 20 5.6 600 0.86 0.39 0.57 1-40m 20 8.7 9/6/2007 2 600 1.09 0.42 0.81 20 8.7 600 1.18 0.87 0.94 1-200-pre 15 5.6 9/6/2007 2 800 0.71 0.55 0.85 15 5.6 800 0.77 0.51 0.74 2-000-pre 20 3 9/6/2007 2 800 0.71 0.55 0.85 20 3 800 0.77 0.51 0.74 2-1000-pre 20 3 8/29/2007 2 600 0.53 2.11 0.22 20 3 600 0.36 3.24 0.27 3-000-pre 20 5.2 8/22/2007 2 600 1 0.85 0.61 20 5.2 600 1.17 2.37 0.67 3-200-pre 20 8.3 8/22/2007 2 600 0.47 0.62 0.52 20 8.3 600 0.48 0.5 0.41 4-000-pre 20 8.4 10/9/2007 2 600 0.94 0.73 0 20 8.4 600 0.93 0.65 0 4-065-pre 20 5 8/17/2007 2 600 1.34 1.57 0.63 20 5 600 0.55 0.46 0.47 5-000-pre 20 3 10/3/2007 2 500 1.3 1.04 1.07 20 3 500 1.17 0.79 0.77 20 3 10/11/2007 1 300 1.19 0.74 0.58 5-043-pre 20 5 10/3/2007 2 500 2.14 1.65 1.38 20 5 500 1.56 1.54 1.16 6-000-pre 20 3.5 1/19/07 (1/17) 3 200 3.99 3.14 3.16 20 3.5 200 4.02 3.28 3.12 20 3.5 200 3.99 3.19 3.08 20 3.5 8/14/2007 2 200 2.51 1.38 2.28 20 3.5 200 3.22 1.82 2.89 7-000-pre (not fractionated) 20 8.3 1/19/07 (1/18) 3 200 4.78 3.43 4.22 20 8.3 200 4.65 3.55 3.83 20 8.3 200 4.07 3.05 4.1 20 8.3 connected 2 200 4.91 3.76 3.95 20 8.3 200 4.99 3.41 3.76 20 8.3 10/8/2007 2 400 2.34 1.9 2.19 20 8.3 400 1.68 1.42 1.57 8-000-pre 20 4 1/10/07 (1/8-10) 3 200 5.14 3.82 5.27 20 4 200 5.38 4.18 4.53 20 4 200 5.62 4.44 4.79 20 4 1/10/07 (1/9) 2 200 4.66 4.07 5.9 20 4 200 5.32 3.69 4.24 20 4 10/9/2007 2 300 1.89 0.88 0.21 20 4 300 2.07 1.09 0.4 9-000-pre 20 6 12/14/06 (12/13) 2 196 4.12 3.29 3.94 20 6 196 4.18 3.19 3.71 10-000-pre 20 5.4 8/14/2007 2 600 1.53 0.26 0.82 vol was 500; changes to 600 20 5.4 600 1.65 0.28 0.83 10-026-pre 20 4.9 8/14/2007 2 600 1.86 1.3 1.04 20 4.9 600 1.03 2.43 0.58 11-000-pre 20 5.5 1/25/07 (1/22) 3 400 2.71 2.43 2.28 20 5.5 400 2.69 2.5 2.23 20 5.5 400 3.38 2.51 2.33 11-026-pre 20 5.5 2/8/07 (2/5) 2 600 3.54 3.72 3.51 20 5.5 600 3.46 3.43 2.94 12-000-pre 20 5 1/25/07 (1/23) 3 400 3.79 3.18 3.17 20 5 400 4.35 3.4 3.64 20 5 400 4.6 3.37 3.5 12-019-pre 20 5.5 2/8/07 (2/7) 2 600 4.33 4.17 4.13 20 5.5 600 4.7 3.69 3.81 7p-000-pre 20 8 12/14/06 (12/11-12)2 200 5.5 3.91 4.74 20 8 200 4.35 3.28 4.22 20 8 10/8/2007 2 400 1.54 0.81 1.43 20 8 400 2.26 1.21 1.89 blank QH20 4.7
Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2 old data GC (Digestion) seawater FR2 Station vol (L) vol (ml) 1-000-pre 20 5.6 1-200-pre 15 5.6
SON 2.34 2.21 2.86 5.72 3.04 2.87 3.04 2.87 0.27 0.34 3.33 4.74 1.58 1.5 0.47 0 0.59 0.47 1.47 1 0.48 1.58 1.48 3.52 3.52 3.75 1.23 1.44 4.88 4.37 4.17 4.85 5.42 0.93 0.68 5.02 4.9 4.89 4.17 4.7 0.64 0.77 3.41 3.61 3.32 3.42 0.68 1 1.91 2.04 2.02 2.26 2.07 2.9 3.35 3.36 3.07 3.58 5.41 3.88 0.58 0.79
SAD 0.67 0.66 0.86 1.75 0.87 0.86 0.87 0.86 0.25 0.25 1.3 1.29 0.56 0.51 1.47 1.26 1.11 0.45 1.31 0.95 1.45 1.66 2.14 2.69 2.67 2.63 1.5 1.85 3.87 3.49 3.67 3.45 3.71 2.24 1.53 3.95 3.81 4.21 3.77 3.79 2.17 2.74 2.32 1.88 1.23 1.33 1.44 3.03 1.52 1.37 1.39 1.54 1.34 2.27 2.51 2.29 2.89 2.83 3.44 2.8 1.44 2.16
CAD 0.22 0.69 0.05 2.09 0.29 0.89 0.29 0.89 5.32 5.22 1.2 2.61 1.26 1.18 1.63 1.52 1.88 0.58 1.76 1.23 1.5 2.8 2.43 4.95 4.59 4.52 1.33 1.72 5.23 5.13 4.73 4.74 5.27 1.61 1.28 5.57 6.1 6.84 6.84 5.76 1.83 2.53 3.93 4.2 1.15 1.22 1.35 2.98 2.7 3.23 3.31 4.34 3.79 4.41 5.17 5.61 5.98 5.74 6.16 5.91 1.16 1.51
total lignin (Λ)
FAD 1.14 1 1.28 2.38 1.48 1.3 1.48 1.3 0.65 0.61 1.34 1.37 0.64 0.63 1.06 1.08 1.15 0.82 1.9 1.24 0.96 1.92 2.03 1.1 1.13 1.17 1.66 1.89 1.43 1.52 1.5 1.29 1.33 1.29 0.91 1.64 1.74 1.98 1.71 1.52 0.89 1.11 1.35 1.13 0.89 0.88 0.78 1.44 0.66 0.8 0.71 1.22 0.87 1.1 1.25 1.11 1.25 1.17 1.63 1.54 0.73 1.01
1 0.9 1.24 1.67 1.3 1.17 1.3 1.17 3.34 3.56 1.31 3.03 1.51 1.33 2.13 2.13 2.53 3.2 1.6 1.18 2 1.94 1.18 1.02 1.15 1.17 2.39 2.57 1.22 1.51 1.56 1.03 1.15 2.27 1.94 1.5 1.44 1.85 1.24 1.26 0.98 1.8 0.75 0.71 2.08 2.06 1.95 1.66 0 0 0 0.41 0.46 1.84 0.97 1.24 1.89 1.57 1.26 0.99 1.43 1.98
S/V
0.7 0.68 1.25 2.41 0.85 0.85 0.34 0.34 0.63 0.69 0.95 1.5 0.99 0.9 1.18 1.06 0.9 0.58 0.69 0.5 0.89 1.51 1.35 4.13 4.11 4.11 2.5 3.05 12.06 11.64 11.14 11.61 12.05 3.06 2.29 6.38 6.42 6.92 6.47 6.06 1.27 1.67 7.07 6.92 1.02 1.05 0.85 1.16 1.95 2.04 2.15 1.88 1.68 2.83 3.08 3.14 2.54 2.48 12.82 10.79 1.82 2.56
C/V 0.14 0.18 0.24 0.46 0.18 0.21 0.07 0.09 0.1 0.08 0.21 0.18 0.29 0.32 0.3 0.25 0.08 0.08 0.08 0.07 0.14 0.12 0.14 0.19 0.18 0.19 0.12 0.11 0.47 0.45 0.46 0.43 0.49 0.15 0.16 0.2 0.21 0.21 0.2 0.22 0.21 0.23 0.26 0.27 0.2 0.19 0.07 0.14 0.11 0.12 0.11 0.07 0.07 0.12 0.12 0.12 0.09 0.09 0.42 0.42 0.17 0.17
total lignin [Ad/Al]v [Ad/Al]s (Λ) S/V 0.09 0.06 0.01 0.69 0.1 0.06 0.03 0.16 0.11 0 1.83 0.2 0.12 0.05 0.12 0.11 0.01 0.85 0.11 0.09 0.03 0.05 0.04 0 0.34 0.05 0.04 0.01 0.07 0.02 1 0.66 0.05 0.04 0.76 0.09 0.05 0.03 1.22 0.09 0.05 0.05 0.18 0.15 0.11 0.95 0.2 0.12 0.11 0.27 0 0.48 1.12 0.28 0 0 0.09 0.04 0.27 0.74 0.23 0.07 0.1 0.06 0.05 0.07 0.59 0.05 0.04 0.07 0.12 0.05 0.32 0.89 0.07 0.06 0.18 1.43 0.08 0.07 0.16 0.04 0.14 0.25 4.12 0.04 0.14 0.23 0.04 0.14 0.21 0.12 0.16 0.19 2.77 0.1 0.16 0.21 0.09 0.37 0.44 11.62 0.1 0.34 0.49 0.11 0.42 0.47 0.08 0.33 0.41 11.83 0.08 0.31 0.4 0.11 0.19 0.36 2.67 0.13 0.19 0.39 0.04 0.2 0.22 6.57 0.05 0.17 0.25 0.05 0.17 0.28 0.04 0.25 0.33 6.27 0.04 0.16 0.25 0.08 0.01 0.38 1.47 0.11 0.03 0.44 0.06 0.29 0.35 7 0.05 0.27 0.36 0.1 0.05 0.03 1.03 0.1 0.05 0.03 0.05 0.05 0.16 1 0.06 0.05 0.24 0.01 0.12 0.19 2.05 0.01 0.11 0.22 0.01 0.09 0.23 0.01 0.09 0.18 1.78 0.01 0.08 0.17 0.04 0.1 0.19 3.02 0.02 0.1 0.19 0.03 0.1 0.21 0.02 0.09 0.18 2.51 0.02 0.07 0.15 0.08 0.34 0.46 11.81 0.09 0.39 0.61 0.11 0.19 0.4 2.19 0.11 0.17 0.38
C/V 0.16
total lignin [Ad/Al]v [Ad/Al]s [Ad/Al]v [Ad/Al]s (Λ) stdev S/V stdev C/V stdev stdev stdev 0.1 0.06 0.02 0.01 0.03 0 0 0.01
0.35
0.18
0.11
0.03
0.82
0.16
0.03
0.01
0.04
0.2
0.12
0.1
0.02
0
0.02
0.01
0.02
0.01
0.08
0.05
0.04
0.01
0
0.01
0
0.01
0.01
0.09
0.06
0.03
0.88
0.04
0.02
0.01
0.01
0.17
0.19
0.09
0.05
0.04
0.39
0.02
0
0
0.01
0.31
0.19
0.13
0.11
0.06
0.02
0.01
0.02
0
0.27
0.28
0
0.24
0.08
0.04
0.01
0
0.34
0.08
0.16
0.05
0.18
0.22
0
0.1
0.02
0.12
0.07
0.06
0.04
0.07
0.13
0.01
0.01
0.01
0
0.14 0.13
0.12 0.08
0.05 0.07
0.32 0.17
0.44 0.11
0.01 0.02
0.03 0
0.01 0.01
0.1 0.01
0.19
0.04
0.14
0.23
0.01
0.01
0
0
0.01
0.11
0.11
0.16
0.2
0.39
0
0.01
0
0.01
0.46
0.1
0.38
0.47
0.29
0.01
0.01
0.02
0.03
0.46
0.08
0.32
0.4
0.31
0.04
0.01
0.01
0
0.15
0.12
0.19
0.37
0.55
0
0.01
0
0.02
0.21
0.05
0.18
0.25
0.03
0
0
0.03
0.02
0.21
0.04
0.21
0.29
0.29
0.01
0
0.07
0.06
0.22
0.1
0.02
0.41
0.28
0.01
0.02
0.01
0.04
0.26
0.05
0.28
0.35
0.11
0.01
0
0.02
0
0.2
0.1
0.05
0.03
0.02
0
0
0
0
0.1
0.06
0.05
0.2
0.22
0.05
0.01
0
0.06
0.12
0.01
0.11
0.21
0.06
0.01
0
0
0.02
0.07
0.01
0.08
0.17
0.14
0
0
0.01
0.01
0.12
0.03
0.1
0.2
0.17
0
0.01
0
0
0.09
0.02
0.08
0.16
0.04
0
0
0.01
0.02
0.42
0.08
0.37
0.53
1.44
0
0
0.03
0.11
0.17
0.11
0.18
0.39
0.52
0
0
0.01
0.01
Analysis year 2006&2007 Each run digested individually units (µg/L) N
VAL
VAD/VAL SAD/SAL VON
VAD
V
SAL
SON
SAD
S
CAD
FAD
C
Prepared by rdv 10/17/2008
total lignin (Λ) S/V
C/V
[Ad/Al]v
[Ad/Al]s
Page 5
9169764.xls 1-integrated 2-000-pre 2-1000-pre 3-000-pre 3-200-pre 4-000-pre 4-065-pre 5-000-pre 5-043-pre 6-000-pre 7-000-pre 8-000-pre
20? ? ? 20 20 20 20 20 20 20 20 20
9-000-pre 10-000-pre 10-026-pre 11-000-pre 11-026-pre 12-000-pre 12-019-pre 7p-000-pre blank QH20
20 20 20 20 20 20 20 20
8.7 ? 3 5.2 8.3* 8.4 2/2/07(2/1) 5 ? 5 3.5* 1/19/07 (1/17) 3 8.3 1/19/07 (1/18) 3 4* 1/10/07 (1/8-10) 3 1/10/07 (1/9) 2 <6 12/14/06 (12/13) 2 5.4 4.9 5.5 1/25/07 (1/22) 3 5.5 2/8/07 (2/5) 2 5* 1/25/07 (1/23) 3 5.5 2/8/07 (2/7) 2 <8 12/14/06 (12/11-12)3 4.7
0.700± 0.003 .56± .01 .547± .0071.81± .01 .63± .02 1.87± .16 1.39± .11 1.68± .08 4.94± .26 1.86± .15 1.08±.05 .83±.06 .97±.08 2.88±.08 .99±.01 0.10± .1 .78± .05 1.01± .23 2.79± .19 .89± .07 1.27± 0.01 .99±.02 1.16±.05 3.43±.06 1.07±0.04
.47± .01 .82± .04 1.53± .08 2.09± .11 .80±.04 1.23±.13 .757± .003 1.26± .15 .6±.1 1.24±.06
1.92± .04 5.47± .3 3.02±.15 2.90± .07 2.96±.01
.199± .006 .20± .02 .93± .02 4.12± .01 .62± .02 .59± .08 1.21±.0911.6± .5 .36±.03 .32±.04 .68±.08 6.6±.3 0.32± .03 .251± .003 .57± .02 6.27±.29 .38±.05 .22±.01 .60±.06 7.0±.1
1.06± .03 1.11± .02 1.05±.03 1.04± .05 .86±.02
.22± .01 .78± .01 .25± .03 .90± .09 .23±.02 .91±.10 .206± .006 1.0± .3 .17±.01 .92±.05
1.3± .1 1.13± .05 1.25±.14 1.4± .3 1.16±.01
0.40± .05 0.321 ± .005 .53± .05 .41 ± .02 1.93±.24
.341± .006.314± .0071.06± .06 .27± .01 0.33 ± .020.30 ± .04.94 ± .06 .20 ± .01 .41± .02 .43± .03 1.38± .09 .40± .03 .36 ± .03 .36 ± .02 1.14 ± .03.30 ± .03 1.42±.13 1.85±.14 5.21±.46 1.76±.35
.20± .01 .42± .05 .13 ± .01 .37 ± .04 .29±.02 .63± .08 .262 ± .004.54 ± .02 1.22±.13 2.44±.06
.89± .04 .10± .01 ND .10 ± .012.05 ± .10 .70 ± .06 .10 ±.02 .040 ±.004 .14 ±.02 1.78 ± .14 1.33± .11 .14± .01 .16± .06 .31± .05 3.02± .16 1.10 ± .01.111 ± .005.16 ± .02 .27 ± .032.51 ±.04 5.43±.51 .65±.04 .42±.08 1.07±.0711.7±1.0
.85 ± .04 .74 ± .02 .96± .02 0.97 ± .04 1.04±.04
.09 ± .01 .79 ± .09 .14 ± .01 .92 ± .10 .23± .05 .81± .04 .24 ± .02 .88 ± .10 0.20±.0.01 .96±.09
1.55 ± .12 1.88 ± .07 1.58± .08 1.78 ± .25 1.41±.24
VON
SAD
FAD
needed to redo to adjust the final volume of py in GC vial!! st8 2 vessels connected to each other for digestion & used 300watts for the microwave
by Lynne
Station 1-000-pre 1-40m 1-200-pre 2-000-pre 2-1000-pre 3-000-pre 3-200-pre 4-000-pre 4-065-pre 5-000-pre
seawate microw r FR2 ave V vol (L) vol (ml) date of anaysis N (ul) VAL 20 5.6 9/6/2007 600 600 20? 8.7 9/6/2007 600 600 15 5.6 9/6/2007 800 800 ? 3 8/29/2007 500 500 ? 3 8/29/2007 600 600 20 5.2 8/22/2007 600 600 20 8.3* 8/22/2007 600 600 20 8.4 10/9/2007 600 600 20 5 8/17/2007 600 600 3 500 20 10/3/2007
3
5-043-pre
20 20
5
10/11/2007 10/3/2007
6-000-pre
20
3.5*
8/14/2007
7-000-pre
20
8.3
10/8/2007
8-000-pre
20
4*
10/9/2007
9-000-pre 10-000-pre
20 20
<6 12/14/06 (12/13) 5.4 8/14/2007
10-026-pre
20
4.9
8/14/2007
11-000-pre
20
5.5
8/7/2007
11-026-pre
20
5.5
8/7/2007
12-000-pre 12-019-pre 7p-000-pre
20 20 20
5* 5.5 <8
10/8/2007
VAD
SAL
SON
CAD
1.05 0.86 1.09 1.18 0.71 0.77 0.53 0.76 0.53 0.36 1 1.17 0.47 0.48 0.94 0.93 1.34 0.55 1.3 1.17 1.19 2.14 1.56 2.51 3.22 2.34 1.68 1.89 2.07
0.43 0.39 0.42 0.87 0.55 0.51 0.18 0.2 2.11 3.24 0.85 2.37 0.62 0.5 0.73 0.65 1.57 0.46 1.04 0.79 0.74 1.65 1.54 1.38 1.82 1.9 1.42 0.88 1.09
0.66 0.57 0.81 0.94 0.85 0.74 0.29 0.36 0.22 0.27 0.61 0.67 0.52 0.41 0 0 0.63 0.47 1.07 0.77 0.58 1.38 1.16 2.28 2.89 2.19 1.57 0.21 0.4
2.34 2.21 2.86 5.72 3.04 2.87 0.41 0.34 0.27 0.34 3.33 4.74 1.58 1.5 0.47 0 0.59 0.47 1.47 1 0.48 1.58 1.48 1.23 1.44 0.93 0.68 0.64 0.77
0.67 0.66 0.86 1.75 0.87 0.86 0.46 0.18 0.25 0.25 1.3 1.29 0.56 0.51 1.47 1.26 1.11 0.45 1.31 0.95 1.45 1.66 2.14 1.5 1.85 2.24 1.53 2.17 2.74
0.22 0.69 0.05 2.09 0.29 0.89 1.1 2.39 5.32 5.22 1.2 2.61 1.26 1.18 1.63 1.52 1.88 0.58 1.76 1.23 1.5 2.8 2.43 1.33 1.72 1.61 1.28 1.83 2.53
1.14 1 1.28 2.38 1.48 1.3 0.73 0.63 0.65 0.61 1.34 1.37 0.64 0.63 1.06 1.08 1.15 0.82 1.9 1.24 0.96 1.92 2.03 1.66 1.89 1.29 0.91 0.89 1.11
1 0.9 1.24 1.67 1.3 1.17 1.69 1.55 3.34 3.56 1.31 3.03 1.51 1.33 2.13 2.13 2.53 3.2 1.6 1.18 2 1.94 1.18 2.39 2.57 2.27 1.94 0.98 1.8
600 500 600 600 200 200 200 200
1.53 1.65 1.86 1.03
0.26 0.28 1.3 2.43
0.82 0.83 1.04 0.58
3.32 3.42 0.68 1
1.23 1.33 1.44 3.03
1.15 1.22 1.35 2.98
0.89 0.88 0.78 1.44
2.08 2.06 1.95 1.66
400 400
1.54 2.26
0.81 1.21
1.43 1.89
0.58 0.79
1.44 2.16
1.16 1.51
0.73 1.01
1.43 1.98
500 300 500 500 200 200 400 400 300 300
by tammi Lignin-derived phenols MARS/ GC analysis MAB Cruise Sept. 2005 C18 SPE extractions FR2
Each run digested individually
GC (Digestion) Station 1-000-pre 1-200-pre 1-integrated
seawater FR2 vol (L) vol (ml) 20 5.6 15 5.6 20? 8.7
units (ng/uL) FR2 vol (ml) N used VAL
VAD/VAL SAD/SAL
VON
VAD
V
SAL
SON
SAD
S
Prepared by rdv 10/17/2008
CAD
FAD
C
total lignin (Λ) S/V
C/V
[Ad/Al]v
[Ad/Al]s
Page 6
9169764.xls 2-000-pre 2-1000-pre 3-000-pre 3-200-pre 4-000-pre 4-065-pre 5-000-pre 5-043-pre 6-000-pre
? ? 20 20 20 20 20 20 20
? 3 5.2 8.3* 8.4 2/2/07(2/1) 5 ? 5 3.5* 1/19/07 (1/17)
3
7-000-pre
20
8.3 1/19/07 (1/18)
3
8-000-pre
20
4* 1/10/07 (1/8-10)
3
1/10/07 (1/9)
2
9-000-pre
20
<6 12/14/06 (12/13)
2
10-000-pre 10-026-pre 11-000-pre
20 20 20
5.4 4.9 5.5 1/25/07 (1/22)
3
11-026-pre
20
5.5 2/8/07 (2/5)
2
12-000-pre
20
12-019-pre
20
5.5 2/8/07 (2/7)
7p-000-pre
20
<8 12/14/06 (12/11-12)3
connected
blank QH20
5* 1/25/07 (1/23)
3
2
200 200 200 200 200 200 200 200 200 200 200 200 200 196 196
3.99 4.02 3.99 4.78 4.65 4.07 4.91 4.99 5.14 5.38 5.62 4.66 5.32 4.12 4.18
3.14 3.28 3.19 3.43 3.55 3.05 3.76 3.41 3.82 4.18 4.44 4.07 3.69 3.29 3.19
3.16 3.12 3.08 4.22 3.83 4.1 3.95 3.76 5.27 4.53 4.79 5.9 4.24 3.94 3.71
3.52 3.52 3.75 4.88 4.37 4.17 4.85 5.42 5.02 4.9 4.89 4.17 4.7 3.41 3.61
2.69 2.67 2.63 3.87 3.49 3.67 3.45 3.71 3.95 3.81 4.21 3.77 3.79 2.32 1.88
4.95 4.59 4.52 5.23 5.13 4.73 4.74 5.27 5.57 6.1 6.84 6.84 5.76 3.93 4.2
1.1 1.13 1.17 1.43 1.52 1.5 1.29 1.33 1.64 1.74 1.98 1.71 1.52 1.35 1.13
1.02 1.15 1.17 1.22 1.51 1.56 1.03 1.15 1.5 1.44 1.85 1.24 1.26 0.75 0.71
400 400 400 600 600 400 400 400 600 600 200 200
2.71 2.69 3.38 3.54 3.46 3.79 4.35 4.6 4.33 4.7 5.5 4.35
2.43 2.5 2.51 3.72 3.43 3.18 3.4 3.37 4.17 3.69 3.91 3.28
2.28 2.23 2.33 3.51 2.94 3.17 3.64 3.5 4.13 3.81 4.74 4.22
1.91 2.04 2.02 2.26 2.07 2.9 3.35 3.36 3.07 3.58 5.41 3.88
1.52 1.37 1.39 1.54 1.34 2.27 2.51 2.29 2.89 2.83 3.44 2.8
2.7 3.23 3.31 4.34 3.79 4.41 5.17 5.61 5.98 5.74 6.16 5.91
0.66 0.8 0.71 1.22 0.87 1.1 1.25 1.11 1.25 1.17 1.63 1.54
0 0 0 0.41 0.46 1.84 0.97 1.24 1.89 1.57 1.26 0.99
4.7
needed to redo to adjust the final volume of py in GC vial!! st8 2 vessels connected to each other for digestion & used 300watts for the microwave
Prepared by rdv 10/17/2008
Page 7
Lignin-derived phenols MARS/ GC analysis MAB Cruise Apr. 2006 C18 SPE extractions FR2 units (µg/L) seawater FR2 Station vol (L) vol (ml) 1-000-pre 20 4.89 1-200-pre 20 4.78 4-000-pre 20 5.34 4-065-pre 20 4.97 5-000-pre 20 4.9 5-043-pre 20 5 10-000-pre 20 4.94 10-026-pre 20 4.95 11-000-pre 20 4.99 11-020-pre 20 4.99 12-000-pre 20 4.92 12-018-pre 20 4.99 b1-28-000-pre 20 4.91 b1-14-000-pre 20 4.95 b2-2-000-1 20 5* b2-2-000-2 20 5.07 b3-0-000-1 20 5.07 b3-0-000-2 20 4.96
GC Analysis Date
needed to redo to adjust the final volume!!
N
VAL
VON
VAD
V
SAL
SON
SAD
S
CAD
FAD
C
total lignin (Λ) S/V
C/V
VAD/VAL SAD/SAL [Ad/Al]v
[Ad/Al]s
Lignin-derived phenols MARS/ GC analysis MAB Cruise Apr. 2006 C18 SPE extractions FR2 units (µg/L) Station 1-000-pre 1-017-pre 2-000-pre 2-026-pre 4-000-pre 4-075-pre 7-000-1-pre 7-000-2-pre 7-1000-1-pre 7-1000-2-pre 8-000-pre 8-200-pre 10-000-pre 10-035-pre 11-000-pre 11-025-pre 12-000-pre 14-000-pre 16-000-1-pre 16-000-2-pre 19-000-1-pre 19-000-2-pre 20-000-pre 21-000-pre
seawater FR2 vol (L) vol (ml) 20 4.398 20 4.218 20 4.199 20 4.199 20 4.348 20 4.298 20 2.215 20 4.298 20 4.247 20 4.137 20 4.226 20 4.108 20 4.327 20 4.284 20 4.218 20 4.199 20 5.399 20 6.643 20 4.624 20 5.610 20 5.395 20 5.447 20 7.925 20 4.994 seater vol check!!
GC Analysis Date
N
VAL
VON
VAD
V
SAL
SON
SAD
S
CAD
FAD
C
total lignin (Λ) S/V
C/V
VAD/VAL SAD/SAL [Ad/Al]v
[Ad/Al]s
Station FR2 volume 101206-B28 5.1 101206-B28B 5.5 101306B26 9.2 101306B26A 9.54 101406MB-A 9.1 101406MB-B 9.25 101406UB-A 10.29 101406UB-B 9.54 101506-B0A 20.84 101506-B0B 10.98 1-1000 3.2 2-000 4.2 2-200 5.1 3-000 6.2 3-030 4.24 4-000 6.26 4-025 6.2 5-000 2.3 5-111 4.2 6-000 6.39 6-021 4.2 7-000 6.4 7-020 4.34
MAB1206 Station # Depth Fr2 Added 2 0 400 2 50 400 3 30 (R1) 500 3 30 (R2) 500 4 20 (R1) 500 4 20 (R2) 500 6 0 500 6 15 500 7 0 (R1) 300 7 0 (R2) 300 8 0 (R1) 300 8 0 (R2) 300 9 0 500 9 20 500 11 0 (R1) 400 11 0 (R2) 400 13 0 300 14 0 (R1) 400 14 0 (R2) 400
MAB Cruise Date Station # Depth FR2 Added MAB0806 19 0 200 MAB0806 8 0 400 MAB0806 11 surface 300 MAB0806 11 deep 300 MAB0905 11 surface 300 MAB0905 11 deep 300 MAB0905 6 0 100 MAB0905 7 original 100 MAB0905 8 0 50 MAB0905 9 0 100
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