Final Khorana Application

APPLICATION FORM FOR KHORANA SUMMER RESEARCH Khorana Scholars Program 2010

1. Name: First name Surname

Sri

Middle name

balaji

Ponraj

2. Male/Female:

Male

3. Date of Birth: 01/09/1986 4. Passport number: g5792123 5. Name of the University: Anna University Coimbatore 6. Address: University Address

Anna University Coimbatore, Academic Campus Jothipuram, Coimbatore - 641 047

Residential Address

East Street, Anaimalaianpatty, Uthamapalayam(Taluk), Theni(District), Tamil nadu(State), India 625 526

Telephone

Landline Number # 04222696341

E-mail

[email protected]

Skype id

bathani01

Mobile # 9894844829

7. Major field of study: Nanotechnology 8. Degree program currently enrolled in: Master of Technology 9. Cumulative Grade Point Average: 9.16 (First year) 10. Expected date of Graduation : June 2010

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11. Academic Record: EDUCATIONAL QUALIFICATIONS (10th Standard onwards) Examination/ Degree

10th Standard

University/ Board

Matriculation

HSCC

State Board

B.Tech

Anna University Chennai

M.Tech

Anna University Coimbatore

Percentage/ CGPA/ Rank

Institute/ College/ School

Duration (from mm/yy to mm/yy)

Vivekananda Matriculation School

June 2001 to March 2002

Science, Mathematics, History and Geography

84.45

June 2003 to March 2004

Physics, Chemistry, Biology and Mathematics

91.25

August 2004 to April 2008

Biotechnology

78.77

August 2008 to June 2010

Nanotechnology

9.16

Theni Kammavar Sangam Matriculation higher Secondary School Arulmigu kalasalingam College of Engineering Anna University Coimbatore

Subjects studied/ Specialization

12. Research interests, possible project ideas and what you hope to gain from this experience. Research interests: Title: 8-OHdG – a novel Biomarker to evaluate Oxidative DNA damage in Micro vascular Complications. Description: Vascular complications are the leading cause of morbidity and mortality in patients with diabetes. In nuclear and mitochondrial DNA, 8-hydroxydeoxyguanosine (8OHdG), an oxidized nucleoside of DNA, is the most frequently detected and studied DNA lesion. Upon DNA repair, 8-OHdG is excreted in the urine. Numerous studies have shown that oxidative DNA damage links pathogenically to a variety of aging-associated degenerative diseases such as cancer, coronary heart disease and diabetes. Quantification of urinary 8-OHdG, a specific DNA repair products in the urine, can be made with a simple ELISA. Finally, the study of Urinary 8-OHdG can lead to predict the earlier onset of vascular complications.

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Project ideas: Title: Synthesis and Characterization of Cerium oxide Nanoparticle to study the hyperglycemia induced Endothelial Dysfunction in Cultured Bovine Retinal Endothelial Cells. Description: In order to combat the hyperglycemia induced Endothelial Dysfunction in humans, I have proposed that rare-earth cerium oxide (CeO2) nanoparticles (free-radical scavengers) protect normal tissue from hyperglycemia induced damage. Earlier studies and reports suggest that these nanoparticles may be a therapeutic regenerative nanomedicine that will scavenge reactive oxygen species, which are responsible for such induced cell damage. In the end, CeO2 nanoparticles provide a tremendous potential for endothelial cell protection and can lead to significant benefits for the preservation of human health and the quality of life for humans having Diabetes Mellitus. Gain from this experience: 1. Gain better understanding of the scientific process and the application of the knowledge gained from basic science research and state of the art techniques to solve complex problems in biology and medicine. 2. Like to be a member of a research community working at the forefront of science, medicine and biotechnology. 13. Research experience, technical background, other internships, courses, lab work, techniques exposed to and past lab experience. Research experience: Completed a project entitled “Induction of Apoptosis by Elevated levels of Glucose through Caspase -3 Activation in Bovine Retinal Endothelial Cells” for a period of four months at Animal Cell Culture Laboratory, Kalasalingam University, Tamil Nadu, India. Technical background: Familiarized with the theoretical concepts and the practical applications of various subjects like Bio-Nanotechnology, Nanolithography, Molecular Biology, Microbiology, Animal Biotechnology, Plant Biotechnology and Immunology. Internships: Completed a project entitled “Strain improvement of Streptomyces.sp to increase production of small molecules- Immunomycin” at Research and Development Department, BIOCON LIMITED, Bangalore, India, from May 15th 2008 to August 16th 2008.

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Courses: 1. Undergone a training programme entitled “Maintenance of Industrially important cells and their Molecular Characterization” organized by PSG College of Technology Coimbatore and Supported by Department of Biotechnology(Ministry of Science and Technology) from November 9th to November 21st 2009. 2. Undergone an Inplant training programme at Quality Control and Production Department, PHARMAFABRIKON Madurai, Tamil Nadu, India, from June 21st to June 27th 2006. 3. International Seminar and Workshop on Medical and Pharmaceutical Nanotechnology held at Anna University Tiruchirappalli, Tamil Nadu, India from September 25th to September 26th 2008. 4. National Workshop on MEMS in Biomedical Applications held at SSN College of Engineering Chennai, Tamil Nadu, India from September 17th to September 18th 2009 . Lab work: PCR-RFLP, RAPD, Multiplex-PCR, Western Blotting, HPLC, Gel Electrophoresis, Atomic Force microscopy, Scanning Tunneling Microscopy, Laser Raman Spectro Photometer, Particle size Analyzer and Particle Spin coater. Techniques exposed to and past lab experience: 1. High Performance Liquid Chromatography Measured the activites of various Anti-Cancer molecules such as Tacrolimus, Orlistat and Hygromycin through HPLC. 2. Cell Cytotoxocity Assays The cells harvested from animal tissues and organs are analyzed for measuring apoptosis. (Caspase and MTT Assay) 3. Maintenance of Industrially Important Cells and their Molecular Characterization of Cells Cryopreservation, Isolation of DNA, PCR-RFLP, RAPD, Multiplex PCR, Western Blotting and PCR-Primer designing Bioinformatics tools. 4. Biochemical estimation on Type-II Diabetic patients Biochemical estimation of Blood (Glucose, Creatinine, Urea and Hydrogen Peroxide) and Urine (Glucose, Urea, Hydrogen Peroxide and Ketone bodies).

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16. Supporting Documentation check-list: Current transcript (Latest Marks sheet)

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Two letters of recommendation from a faculty member, advisor or lab supervisor on their official letterhead

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Statement from Parent Institution (in the format provided in S.No. 14)

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