Extraction Of Adn Human.docx

Paula Andrea Sastoque Rey Grupo IV B A simple method of extracting genomic DNA from human samples for PCRRFLP analysis DNA typing is currently the most validated method for personal identification of human body fluid spots found in crime scenes, the most common method is obtaining genomic DNA from nucleated cells of peripheral blood. We can also isolate DNA including buccal cells, follicle hair and urine, which are easier to obtain in a non-invasive way than by an invasive blood collection. The collection of these samples can be easily performed as the oral cell collection by a cotton swab or mouthwash offering many advantages such as cost-effective processing, the requirement of smaller sample volume, long-term archiving and suitability of self-collection. Human hair is one of the biological materials most associated with legal research. The most valuable method of DNA testing is the short tandem repeat analysis of nuclear DNA, which is possible when the portion of the hair root or adhered tissue is present. The proteins in the hair samples require additional steps to break the stem and release the DNA, thus exposing the sample to a higher risk of contamination. Human urine is an adequate sample for toxicological analysis in doping tests and drug detection. Demographic information, age, health status, sex, ancestry of the population, hair color and hair treatments were collected in five adult volunteers between 22-35 years of age, urine samples were also collected in vials of samples sterile and blood samples from the same donors by capillary puncture. The yield of the DNA extracted from the four different sample sources was evaluated using a UV-visible double-beam spectrophotometer and gel electrophoresis, for the verification of DNA quality the D-loop mtDNA region was amplified by an assay of PCR. A reliable PCR reaction can be performed using a much smaller amount of the isolated DNA. The intensity of the PCR product was higher for the DNA of the blood and hair template than with the swab and urine samples. The degradation of the DNA bands was observed in buccal swabs and in urine samples processed with delay, while no degradation was observed in blood and hair samples. The simple and novel method of sample collection and DNA extraction, which is cost-effective, easy and fast, was demonstrated, providing a sufficient quantity and quality of DNA for PCR-RFLP-based analysis. The simple phenol-chloroform method is the most suitable for the extraction of DNA from samples of buccal swab, urine, hair and blood. References:

Nihgov. PubMed Central (PMC). [Online]. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792701/ [Accessed 18 March 2019].