Dushyant Abt201 Final

Plant antibiotic production through plant tissue culture

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DUSHYANT KUMAR BSA-08-613

Antibiotics These are the chemical compounds which are

having the property act against the biological disease producing agents likely bacteria, virus and fungus. Example of antibiotics Antibiotic Quinine Barberin Artemisin Ajmalicin

plant spp. cinchona Papaver somniferum Artimesia spp. Cartharanthus roseus

Steps of production Production strategy consist of two distinct phase Growth phase for cell biomass accumulation Production phase for biosynthesis and accumulation of biochemical (antibiotic)

Nutrient medium Linsmaier and skoog medium (LS medium). It contain Basal salt mixture containing micro and

macro elements with vitamins . All high value biochemicals from cultured plant cells are secondary metabolities. Biochemicals production by plant cells is markedly influenced by the constituent of culture medium & temp.,light,inoculum size.

Improving bio chemicals production Suitable culture medium & conditions. Development of high producing cultures. Use of elicitors. Use of organ cultures.

Biochemical content of cell cultures depends on

the plant species the genotype or strain of the species . Cell cultures are highly heterogeneous for biochemical production in that different cells shows different levels of production . This variation is used to advantage by screening a large nu. Of clones for isolation of high producing clones

Often the high yielding clones so isolated exhibit a

decline in production levels on being maintained by serial subculture but in several cases stable high producing clones have been successfully isolated. For production of biochemicals from plant cell cultures since it greatly reduces the cost of production.

PREPARATION OF PLANT TISSUE CULTURE MEDIUM Measure approximately 90% of the required

volume of the de ionized-distilled water in a flask/container of double the size of the required volume. Add the dehydrated medium into the water and stir to dissolve the medium completely. Gentle heating of the solution may be required to bring powder into solution

Add desired heat stable supplements to the medium

solution. Add additional de ionized-distilled water to the medium solution to obtain the final required volume. Set the desired pH with NaOH or HCl. Dispense the medium into culture vessels

Sterilize the medium by autoclaving at 15 psi (121°C)

for appropriate time period. Higher temperature may result in poor cell growth. Add heat labile supplements after autoclaving.