Data Pengamatan Absorpsi Oral.docx

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7. DATA PENGAMATAN 7.1 Data penentuan kadar obat secara in vitro Nama bahan Obat : Caffein 0,01M Cairan serosal

: NaCl 0,9%

Volume : 1,4 ml

Medium cairan mucosal : Dapar HCl 0,2N

Volume : 75 ml

Dapar KCl 0,2M

Volume : 75 ml

7.2 Data penentuan kadar obat secara spektrofotometri Pengambilan larutan sampel pada menit : 0,5,10,15,20,25,30,35,40,45 Percobaan dilakukan pada λ maks = 271 nm Persamaan garis kurva baku Caffein dengan dapar HCl (pH 1,2) : y = 0,0047x - 0,0404 R² = 0,9977 Persamaan garis kurva baku Caffein dengan dapar KCl (pH 7,4) : y = 0,1056x + 0,025 R² = 0,9704

7.3 Media Dapar KCl 0,2M Tabel 7.1 Absorpsi Obat dengan Media Dapar KCl 0,2M

Waktu 0 5 10 15 20 25 30 35 40 45

Mg terabsorpsi 4,762 5,224 5,735 6,086 6,278 6,629 8,288 8,591 8,927 9,134

Dapar Fosfat (KCl) % Terabsorpsi Permeabilitas 3,277 3,6 3,957 4,204 4,342 0,055103 4,589 5,738 5,954 6,193 6,345

Lag Time (menit)

43,10945

7.4 Media Dapar HCl 0,2N Tabel 7.2 Absorpsi Obat dengan Media Dapar HCl 0,2N Dapar HCl Waktu 0 5 10 15 20 25 30 35 40 45

Mg terabsorpsi 0,374 0,418 0,472 0,496 0,548 0,621 0,985 1,329 1,553 2,009

% Terabsorpsi Permeabilitas 0,257 0,288 0,326 0,343 0,379 0,017938144 0,429 0,681 0,918 1,074 1,389

Lag Time (menit)

2,9598

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