Biologi K3 Percubaan Sbp 2007

SULIT

4551/3

3

Answer all questions. 1. A group of students carried out an experiment to study the anaerobic respiration in yeast. Diagram 1.1 shows the set-up of the experiment.

Delivery tube

Delivery tube Thermometers

Stoppers Boiling tubes

Test tube A

Test tube B

Paraffin oil

Lime water Set A

Lime water Set B

5 cm3 yeast suspension + 5 cm3 boiled glucose solution

5 cm3 distilled water + 5 cm3 boiled glucose solution

Diagram 1.1 The thermometer readings and the observations on the lime water were taken at 0 minute and 30 minutes. Diagram 1.2 shows the observations made on the lime water.

Lime water (clear)

4551/3

Lime water (chalky)

SULIT

SULIT

4551/3

4

Diagram 1.2 Table 1.3 shows the result of the experiment at 0 minute and 30 minutes. Time: 0 minute Set A

Set B

40

ºC

30

30

20

20

Thermometer reading

ºC

40

Thermometer reading 29 ºC

31 ºC

Time: 30 minutes Set A

Set B

40

ºC

40

30

30

20

20

Thermometer reading 37 ºC

ºC

Thermometer reading 29 ºC

Table 1.3

4551/3

SULIT

SULIT

5

4551/3 For examiner’s use

(a) State the thermometer readings in the spaces provided in Table 1.3.

[3 marks]

1 (a) For examiner’s use

(b) (i) Based on Table 1.3, state two observations that can be made on Set A in the experiment. [3 marks] Observation 1 on Set A: At 30 minutes, the thermometer reading is 37 ºC, higher than at time 0 minute / has increased.

Observation 2 on Set A: At 0 minutes, the lime water has not changed into chalky, compared to 30 minutes.

(ii) State the inference for each observation made in (b) (i).

[3 marks]

Inference for observation 1 on Set A: At 30 minutes, the temperature increased because heat was produced from the respiration of yeast / anaerobic respiration of yeast.

Inference for observation 2 on Set A: At 0 minutes, the CO2 content in the lime water had not increased from the respiration of yeast / anaerobic respiration of yeast.

(c) Complete Table 1.4 to show the variables involved in the experiment and how the variables are operated. [3 marks] 4551/3

SULIT

1 (b) (i)

1 (d) (c)

SULIT

Variables Manipulated variable: The presence of yeast

………………………….... ……………………………

4551/3

6

1 (c)

Operating the variables How to alter the manipulated variable: Set A with yeast but Set B without yeast

………………………………..…………………… ……………………………………………………

Responding variable:

How to determine the responding variable:

Production of heat /

Recording the thermometer reading in ºC, and

increase in temperature,

the change in lime water.

and production of CO2 /

………………………………..……………………

change in lime water

How to maintain the controlled variable: Controlled variable:

Using the same volume / concentration of

Volume / concentration of boiled glucose solution // Anaerobic condition

boiled glucose solution // Anaerobic condition (for both sets)

……………………………………………… Table 1.4 (d) State the hypothesis for this experiment. Yeast (V1) carries out anaerobic respiration (R) which produced heat / energy and carbon dioxide (V2).

……………………………………………………………………………………… [3 marks] (e) (i) Construct a table and record the results of the experiment. [3 marks]

1 (e) (i)

4551/3

SULIT

SULIT

4551/3

7

(ii) Explain the relationship between the boiled glucose solution and paraffin oil used in the experiment of the anaerobic respiration process in yeast. …………………..…………..…………………………………………………... ……………………………………………………………………………………

For examiner’s use

For examiner’s use

1 (e) (ii)

…………………………………………………………………………………… [3 marks] (f) Following are some of the apparatus and materials used in the experiment.

Yeast suspension, boiled glucose solution, distilled water, thermometers, lime water, and paraffin oil

. Categorise the apparatus and materials according to their roles in operating the variables in the experiment. [3 marks]

1 (f)

(g) Based on the experiment, define operationally anaerobic respiration. ……………………………………………………………………………………… ……………………………………………………………………………………… ……………………………………………………………………………………… [3 marks]

4551/3

SULIT

1 (g)

SULIT

8

4551/3

(h) The experiment is repeated by using Set C containing 10 cm3 of yeast suspension and 10 cm3 of boiled glucose solution. (i) Predict the readings on the thermometer used in the experiment. Explain your answer. ……..………..…………..……………………………………………………….. ……………………………………………………………………………………

1 (h) (i)

…………………..………….. …………………………………………………………………………………… …………………………………………………… [3 marks] (ii) Explain the time taken for the lime water to change. …………………..…………..…………………………………………………… …………………………………………………………………………………… …………………..…………..…………………………………………………… [3 marks]

4551/3

SULIT

1 (h) (i)

SULIT

9

4551/3

2. Aquatic plants carry out photosynthesis to obtain food. In the process, oxygen bubbles are released to the aquatic environment. The rate of photosynthesis is affected by several environmental factors such as temperature, light intensity and concentration of carbon dioxide.

Design an experiment to determine the effect of carbon dioxide concentration on the rate of photosynthesis. (17 marks)

END OF QUESTION PAPER

4551/3

SULIT

SULIT

10

4551/3

SPACE FOR ANSWERS Section:..............................................

For examiner’s use

Question no:.............................................

_____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________

4551/3

SULIT

SULIT

11

4551/3

SPACE FOR ANSWERS Section:..............................................

For examiner’s use

Question no:.............................................

_____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________

4551/3

SULIT

SULIT

12

4551/3

SPACE FOR ANSWERS Section:..............................................

For examiner’s use

Question no:.............................................

_____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________

4551/3

SULIT

SULIT

13

4551/3

SPACE FOR ANSWERS Section:..............................................

For examiner’s use

Question no:.............................................

_____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________ _____________________________________________________________________

4551/3

SULIT