0102 Final

We have covered the yeast two-hybrid system in the class. However, we have not discussed the yeast one-hybrid system that is actually a simpler system allowing us to isolate DNA binding proteins. Based on your knowledge on yeast two-hybrid system and the hints given below, explain how you can use yeast one-hybrid system to identify proteins specifically binding to a target DNA segment. [Hint: many activation domains can initiate transcription so long as it is placed near the RNA polymerase; yeast one-hybrid does not require the interaction of two proteins] (8 marks) Question 2 VNTR is used by FBI as evidence to prosecute a criminal suspect. Explain the principle of VNTR and why it is suitable for this purpose (5 marks). Question 3 A scientist decides to study genes that control the flowering mechanism of a plant. He has three graduate students. He asked one student to make DNA chips from a flower library, another student to construct genetic and physical map of the target plant, and the remaining student to generate flowering mutants. Explain the overall strategies used by this scientist (10 marks) Question 4 Distinguish the terms differential expression, differential display and differential hybridization (6 marks) Question 5 Answer either (a) or (b) of the following questions (6 marks) (a)

State any one good question asked during the BIO4320 student presentations. Explain why you think this question is good and give an answer to this question. [Questions asked in your presentation cannot be used. However, questions asked by yourself in other student’s presentation is acceptable.]

(b)

Within the topics covered in Dr. Lam’s lecturing materials, state one question you think that should have (but not) appeared in this examination. Explain why this question is so important and provide a brief answer for it.

5. Human embryonic stem cells are totipotent with potential application in medicine. Describe and discuss the following potential application: therapeutic cloning. (3 marks) - THE END -